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Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against IschemiaReperfusion Injury
المؤلفون المشاركون
Csont, Tamás
Pálóczi, János
Varga, Zoltán V.
Apáti, Ágota
Szebényi, Kornélia
Sarkadi, Balázs
Madonna, Rosalinda
De Caterina, Raffaele
Eschenhagen, Thomas
Ferdinandy, Péter
Görbe, Anikó
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-15
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background and Aims.
Human embryonic stem cell- (hESC-) derived cardiomyocytes are one of the useful screening platforms of potential cardiocytoprotective molecules.
However, little is known about the behavior of these cardiomyocytes in simulated ischemia/reperfusion conditions.
In this study, we have tested the cytoprotective effect of an NO donor and the brain type natriuretic peptide (BNP) in a screening platform based first on differentiated embryonic bodies (EBs, 6 + 4 days) and then on more differentiated cardiomyocytes (6 + 24 days), both derived from hESCs.
Methods.
Both types of hESC-derived cells were exposed to 150 min simulated ischemia, followed by 120 min reperfusion.
Cell viability was assessed by propidium iodide staining.
The following treatments were applied during simulated ischemia in differentiated EBs: the NO-donor S-nitroso-N-acetylpenicillamine (SNAP) (10−7, 10−6, and 10−5 M), BNP (10−9, 10−8, and 10−7 M), and the nonspecific NO synthase inhibitor Nω-nitro-L-arginine (L-NNA, 10−5 M).
Results.
SNAP (10−6, 10−5 M) significantly attenuated cell death in differentiated EBs.
However, simulated ischemia/reperfusion-induced cell death was not affected by BNP or by L-NNA.
In separate experiments, SNAP (10−6 M) also protected hESC-derived cardiomyocytes.
Conclusions.
We conclude that SNAP, but not BNP, protects differentiated EBs or cardiomyocytes derived from hESCs against simulated ischemia/reperfusion injury.
The present screening platform is a useful tool for discovery of cardiocytoprotective molecules and their cellular mechanisms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pálóczi, János& Varga, Zoltán V.& Apáti, Ágota& Szebényi, Kornélia& Sarkadi, Balázs& Madonna, Rosalinda…[et al.]. 2016. Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113915
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pálóczi, János…[et al.]. Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113915
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pálóczi, János& Varga, Zoltán V.& Apáti, Ágota& Szebényi, Kornélia& Sarkadi, Balázs& Madonna, Rosalinda…[et al.]. Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113915
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113915
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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