TGF-β and HypoxiaReoxygenation Promote Radioresistance of A549 Lung Cancer Cells through Activation of Nrf2 and EGFR
المؤلفون المشاركون
Lee, Sae-lo-oom
Ryu, Hwani
Son, A-rang
Seo, Bitna
Kim, Jooyoung
Jung, Seung-Youn
Song, Jie-Young
Hwang, Sang-Gu
Ahn, Jiyeon
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-20
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Although many studies have examined the roles of hypoxia and transforming growth factor- (TGF-) β separately in the tumor microenvironment, the effects of simultaneous treatment with hypoxia/reoxygenation and TGF-β on tumor malignancy are unclear.
Here, we investigated the effects of redox signaling and oncogenes on cell proliferation and radioresistance in A549 human lung cancer cells in the presence of TGF-β under hypoxia/reoxygenation conditions.
Combined treatment with TGF-β and hypoxia activated epidermal growth factor receptor (EGFR) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a redox-sensitive transcription factor.
Interestingly, Nrf2 knockdown suppressed the effects of combined treatment on EGFR phosphorylation.
In addition, blockade of EGFR signaling also suppressed induction of Nrf2 following combined treatment with hypoxia and TGF-β, indicating that the combined treatment induced positive crosstalk between Nrf2 and EGFR.
TGF-β and hypoxia/reoxygenation increased the accumulation of reactive oxygen species (ROS), while treatment with N-acetyl-l-cysteine abolished the activation of Nrf2 and EGFR.
Treatment with TGF-β under hypoxic conditions increased the proliferation of A549 cells compared with that after vehicle treatment.
Moreover, cells treated with the combined treatment exhibited resistance to ionizing radiation (IR), and knockdown of Nrf2 increased IR-induced cell death under these conditions.
Thus, taken together, our findings suggested that TGF-β and hypoxia/reoxygenation promoted tumor progression and radioresistance of A549 cells through ROS-mediated activation of Nrf2 and EGFR.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Sae-lo-oom& Ryu, Hwani& Son, A-rang& Seo, Bitna& Kim, Jooyoung& Jung, Seung-Youn…[et al.]. 2016. TGF-β and HypoxiaReoxygenation Promote Radioresistance of A549 Lung Cancer Cells through Activation of Nrf2 and EGFR. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114136
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Sae-lo-oom…[et al.]. TGF-β and HypoxiaReoxygenation Promote Radioresistance of A549 Lung Cancer Cells through Activation of Nrf2 and EGFR. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1114136
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Sae-lo-oom& Ryu, Hwani& Son, A-rang& Seo, Bitna& Kim, Jooyoung& Jung, Seung-Youn…[et al.]. TGF-β and HypoxiaReoxygenation Promote Radioresistance of A549 Lung Cancer Cells through Activation of Nrf2 and EGFR. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114136
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114136
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر