![](/images/graphics-bg.png)
Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro
المؤلفون المشاركون
Pääsuke, M.
Märtson, Aare
Pääsuke, Reedik
Eimre, Margus
Piirsoo, Andres
Peet, Nadežda
Laada, Liidia
Kadaja, Lumme
Roosimaa, Mart
Seppet, Enn
Paju, Kalju
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation.
The aim of the study was to characterize the function of oxidative phosphorylation (OXPHOS), glycolysis, adenylate kinase (AK), and creatine kinase (CK) mediated systems in young and older individuals.
Materials and Methods.
Myoblasts were cultivated from biopsies taken by transcutaneous conchotomy from vastus lateralis muscle in young (20–29 yrs, n=7) and older (70–79 yrs, n=7) subjects.
Energy metabolism was assessed in passages 2 to 6 by oxygraphy and enzyme analysis.
Results.
In myoblasts of young and older subjects the rate of OXPHOS decreased during proliferation from passages 2 to 6.
The total activities of CK and AK decreased.
Myoblasts of passage 2 cultivated from young muscle showed higher rate of OXPHOS and activities of CK and AK compared to myoblasts from older subjects while hexokinase and pyruvate kinase were not affected by ageing.
Conclusions.
Proliferation of myoblasts in vitro is associated with downregulation of OXPHOS and energy storage and transfer systems.
Ageing in vivo exerts an impact on satellite cells which results in altered metabolic profile in favour of the prevalence of glycolytic pathways over mitochondrial OXPHOS of myoblasts.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pääsuke, Reedik& Eimre, Margus& Piirsoo, Andres& Peet, Nadežda& Laada, Liidia& Kadaja, Lumme…[et al.]. 2016. Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114317
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pääsuke, Reedik…[et al.]. Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114317
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pääsuke, Reedik& Eimre, Margus& Piirsoo, Andres& Peet, Nadežda& Laada, Liidia& Kadaja, Lumme…[et al.]. Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114317
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114317
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)