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Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status
المؤلفون المشاركون
Székács, András
Balla, Peter
Pászti-Gere, Erzsébet
Jerzsele, Á.
Ujhelyi, G.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-28
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objectives.
The relationship among matriptase function, cellular redox status, and maintenance of intestinal barrier integrity has not been established yet.
The aim of this study is to reveal if the crosstalk between matriptase activators and intestinal epithelial monolayers can lead to perturbations in physiological redox regulation in vitro.
Methods.
The effects of suramin and sphingosine-1-phosphate (S1P) were tested on viability of intestinal porcine epithelial IPEC-J2 cells using MTS assay.
Measurements of transepithelial electrical resistance (TER) were performed to determine changes in barrier integrity of cell monolayers.
Amplex Red assay was used to monitor extracellular hydrogen peroxide production.
Occludin distribution pattern was detected prior to and after matriptase activation using immunofluorescent staining technique.
Results.
TER reduction was observed in suramin-treated IPEC-J2 cell monolayers, which could be attributed to cell cytotoxic properties of 48 hr 50 μM suramin administration.
In contrast, S1P treatment increased TER significantly and elevated occludin accumulation in tight junctions.
It was also found that extracellular hydrogen peroxide levels were maintained in IPEC-J2 cells exposed to matriptase activators.
Discussion.
S1P administration not accompanied by redox imbalance might be one of the key strategies in the improvement of barrier function and consequently in the therapy of intestinal inflammations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pászti-Gere, Erzsébet& Jerzsele, Á.& Balla, Peter& Ujhelyi, G.& Székács, András. 2015. Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1114802
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pászti-Gere, Erzsébet…[et al.]. Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1114802
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pászti-Gere, Erzsébet& Jerzsele, Á.& Balla, Peter& Ujhelyi, G.& Székács, András. Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1114802
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114802
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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