Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells
المؤلفون المشاركون
Hewitt, Alex W.
Hernández, Damián
Millard, Rodney
Sivakumaran, Priyadharshini
Wong, Raymond C. B.
Crombie, Duncan E.
Liang, Helena
Hung, Sandy S. C.
Pébay, Alice
Shepherd, Robert K.
Dusting, Gregory J.
Lim, Shiang Y.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-14
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Background.
Human induced pluripotent stem cells (iPSCs) are an attractive source of cardiomyocytes for cardiac repair and regeneration.
In this study, we aim to determine whether acute electrical stimulation of human iPSCs can promote their differentiation to cardiomyocytes.
Methods.
Human iPSCs were differentiated to cardiac cells by forming embryoid bodies (EBs) for 5 days.
EBs were then subjected to brief electrical stimulation and plated down for 14 days.
Results.
In iPS(Foreskin)-2 cell line, brief electrical stimulation at 65 mV/mm or 200 mV/mm for 5 min significantly increased the percentage of beating EBs present by day 14 after plating.
Acute electrical stimulation also significantly increased the cardiac gene expression of ACTC1, TNNT2, MYH7, and MYL7.
However, the cardiogenic effect of electrical stimulation was not reproducible in another iPS cell line, CERA007c6.
Beating EBs from control and electrically stimulated groups expressed various cardiac-specific transcription factors and contractile muscle markers.
Beating EBs were also shown to cycle calcium and were responsive to the chronotropic agents, isoproterenol and carbamylcholine, in a concentration-dependent manner.
Conclusions.
Our results demonstrate that brief electrical stimulation can promote cardiac differentiation of human iPS cells.
The cardiogenic effect of brief electrical stimulation is dependent on the cell line used.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hernández, Damián& Millard, Rodney& Sivakumaran, Priyadharshini& Wong, Raymond C. B.& Crombie, Duncan E.& Hewitt, Alex W.…[et al.]. 2015. Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116113
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hernández, Damián…[et al.]. Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116113
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hernández, Damián& Millard, Rodney& Sivakumaran, Priyadharshini& Wong, Raymond C. B.& Crombie, Duncan E.& Hewitt, Alex W.…[et al.]. Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116113
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116113
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر