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Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro
المؤلفون المشاركون
Dottori, Mirella
Crombie, Duncan E.
Gunewardene, Niliksha
Nayagam, Bryony A.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-07
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Induced pluripotent stem cells (iPSCs) may serve as an autologous source of replacement neurons in the injured cochlea, if they can be successfully differentiated and reconnected with residual elements in the damaged auditory system.
Here, we explored the potential of hiPSC-derived neurons to innervate early postnatal hair cells, using established in vitro assays.
We compared two hiPSC lines against a well-characterized hESC line.
After ten days’ coculture in vitro, hiPSC-derived neural processes contacted inner and outer hair cells in whole cochlear explant cultures.
Neural processes from hiPSC-derived neurons also made contact with hair cells in denervated sensory epithelia explants and expressed synapsin at these points of contact.
Interestingly, hiPSC-derived neurons cocultured with hair cells at an early stage of differentiation formed synapses with a higher number of hair cells, compared to hiPSC-derived neurons cocultured at a later stage of differentiation.
Notable differences in the innervation potentials of the hiPSC-derived neurons were also observed and variations existed between the hiPSC lines in their innervation efficiencies.
Collectively, these data illustrate the promise of hiPSCs for auditory neuron replacement and highlight the need to develop methods to mitigate variabilities observed amongst hiPSC lines, in order to achieve reliable clinical improvements for patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gunewardene, Niliksha& Crombie, Duncan E.& Dottori, Mirella& Nayagam, Bryony A.. 2016. Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116129
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gunewardene, Niliksha…[et al.]. Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116129
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gunewardene, Niliksha& Crombie, Duncan E.& Dottori, Mirella& Nayagam, Bryony A.. Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116129
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116129
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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