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Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels
المؤلفون المشاركون
Zandieh-Doulabi, Behrouz
Kleverlaan, Cornelis J.
Klein-Nulend, Jenneke
van Esterik, Fransisca A. S.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-28
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
For bone tissue engineering synthetic biphasic calcium phosphate (BCP) with a hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) ratio of 60/40 (BCP60/40) is successfully clinically applied, but the high percentage of HA may hamper efficient scaffold remodelling.
Whether BCP with a lower HA/β-TCP ratio (BCP20/80) is more desirable is still unclear.
Vascular development is needed before osteogenesis can occur.
We aimed to test the osteogenic and/or vasculogenic differentiation potential as well as degradation of composites consisting of human adipose stem cells (ASCs) seeded on BCP60/40 or BCP20/80 incorporated in fibrin gels that trigger neovascularization for bone regeneration.
ASC attachment to BCP60/40 and BCP20/80 within 30 min was similar (>93%).
After 11 days of culture BCP20/80-based composites showed increased alkaline phosphatase activity and DMP1 gene expression, but not RUNX2 and osteonectin expression, compared to BCP60/40-based composites.
BCP20/80-based composites also showed enhanced expression of the vasculogenic markers CD31 and VEGF189, but not VEGF165 and endothelin-1.
Collagen-1 and collagen-3 expression was similar in both composites.
Fibrin degradation was increased in BCP20/80-based composites at day 7.
In conclusion, BCP20/80-based composites showed enhanced osteogenic and vasculogenic differentiation potential compared to BCP60/40-based composites in vitro, suggesting that BCP20/80-based composites might be more promising for in vivo bone augmentation than BCP60/40-based composites.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
van Esterik, Fransisca A. S.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Klein-Nulend, Jenneke. 2016. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
van Esterik, Fransisca A. S.…[et al.]. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
van Esterik, Fransisca A. S.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Klein-Nulend, Jenneke. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116157
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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