Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels
المؤلفون المشاركون
Zandieh-Doulabi, Behrouz
Kleverlaan, Cornelis J.
Klein-Nulend, Jenneke
van Esterik, Fransisca A. S.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-28
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
For bone tissue engineering synthetic biphasic calcium phosphate (BCP) with a hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) ratio of 60/40 (BCP60/40) is successfully clinically applied, but the high percentage of HA may hamper efficient scaffold remodelling.
Whether BCP with a lower HA/β-TCP ratio (BCP20/80) is more desirable is still unclear.
Vascular development is needed before osteogenesis can occur.
We aimed to test the osteogenic and/or vasculogenic differentiation potential as well as degradation of composites consisting of human adipose stem cells (ASCs) seeded on BCP60/40 or BCP20/80 incorporated in fibrin gels that trigger neovascularization for bone regeneration.
ASC attachment to BCP60/40 and BCP20/80 within 30 min was similar (>93%).
After 11 days of culture BCP20/80-based composites showed increased alkaline phosphatase activity and DMP1 gene expression, but not RUNX2 and osteonectin expression, compared to BCP60/40-based composites.
BCP20/80-based composites also showed enhanced expression of the vasculogenic markers CD31 and VEGF189, but not VEGF165 and endothelin-1.
Collagen-1 and collagen-3 expression was similar in both composites.
Fibrin degradation was increased in BCP20/80-based composites at day 7.
In conclusion, BCP20/80-based composites showed enhanced osteogenic and vasculogenic differentiation potential compared to BCP60/40-based composites in vitro, suggesting that BCP20/80-based composites might be more promising for in vivo bone augmentation than BCP60/40-based composites.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
van Esterik, Fransisca A. S.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Klein-Nulend, Jenneke. 2016. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
van Esterik, Fransisca A. S.…[et al.]. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
van Esterik, Fransisca A. S.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Klein-Nulend, Jenneke. Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116157
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116157
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر