![](/images/graphics-bg.png)
Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner
المؤلفون المشاركون
Kim, Song Cheol
Lee, Song
Jeong, Seonghee
Lee, Chanmi
Oh, Jooyun
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-18
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, and most connective tissues have been recognized as promising sources for cell-based therapies.
MSCs have also been detected in human pancreatic tissue, including endocrine and exocrine cells.
These adult human pancreas-derived MSCs have generated a great deal of interest owing to their potential use in the differentiation of insulin-producing cells for diabetes treatment.
In the present study, we isolated MSCs from the adult human exocrine pancreas to determine whether isolated MSCs have the potential to differentiate into pancreatic endocrine cells and, therefore, whether they can be used in stem cell-based therapies.
Pancreatic tissue was digested by collagenase and an enriched exocrine-cell fraction was obtained by density-gradient separation.
Crude exocrine cells were methodically cultured in suspension and then in adherent culture.
We expanded the human pancreatic exocrine-derived MSCs (hpMSCs) by cell passaging in culture and confirmed by flow cytometry that >90% expressed human classic surface markers of MSCs.
Interestingly, these cells expressed pancreatic transcription factors, such as Pdx1, Ngn3, and MafA, similar to pancreatic progenitor cells.
These results indicated that hpMSCs can be used for the differentiation of pancreatic endocrine cells and may be used in type 1 diabetes treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Song& Jeong, Seonghee& Lee, Chanmi& Oh, Jooyun& Kim, Song Cheol. 2016. Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116193
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Song…[et al.]. Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116193
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Song& Jeong, Seonghee& Lee, Chanmi& Oh, Jooyun& Kim, Song Cheol. Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116193
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116193
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)