Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression
المؤلفون المشاركون
de Carvalho, Katherine Athayde Teixeira
Irioda, Ana Carolina
Cassilha, Rafael
Zocche, Larissa
Francisco, Julio Cesar
Cunha, Ricardo Correa
Ferreira, Priscila Elias
Guarita-Souza, Luiz Cesar
Ferreira, Reginaldo Justino
Mogharbel, Bassam Felipe
Garikipati, Venkata Naga Srikanth
Souza, Daiany
Beltrame, Mirian Perlingeiro
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-15
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
Aim.
The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies.
The aim of this study was to analyze human adipose tissue-derived mesenchymal stem cells phenotypic expression (CD34, CD45, CD73, CD90, CD105, and CD49d), colony forming unit ability, viability, and differentiation potential before and after cryopreservation.
Materials and Methods.
12 samples of the adipose tissue were collected from a healthy donor using the liposuction technique.
The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2.
Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively.
Results.
The immunophenotypic markers expression was largely preserved, and their multipotency was maintained.
However, after cryopreservation, the cells decreased α4-integrin expression (CD49d), cell viability, and number of colony forming units.
Conclusions.
These findings suggest that ADMSC transplanted after cryopreservation might compromise the retention of transplanted cells in the host tissue.
Therefore, further studies are warranted to standardize protocols related to cryopreservation to attain full benefits of stem cell therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Irioda, Ana Carolina& Cassilha, Rafael& Zocche, Larissa& Francisco, Julio Cesar& Cunha, Ricardo Correa& Ferreira, Priscila Elias…[et al.]. 2016. Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116256
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Irioda, Ana Carolina…[et al.]. Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116256
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Irioda, Ana Carolina& Cassilha, Rafael& Zocche, Larissa& Francisco, Julio Cesar& Cunha, Ricardo Correa& Ferreira, Priscila Elias…[et al.]. Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116256
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116256
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر