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The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells
المؤلفون المشاركون
Chen, Ji
Chen, Yanfang
Simman, Richard
Chen, Su-Hong
Lv, Gui-Yuan
Yang, Yi
Wang, Jinju
Guo, Runmin
Jacobs, Bradley
Iwuchukwu, Ifeanyi
Gaines, Kenneth J.
Wu, Keng
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-28
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Exosomes (EXs) are cell-derived vesicles that mediate cell-cell communication and could serve as biomarkers.
Here we described novel methods for purification and phenotyping of EXs released from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads and fluorescence quantum dots (Q-dots®) techniques.
EXs from the culture medium of ECs and EPCs were isolated and detected with cell-specific antibody conjugated microbeads and second antibody conjugated Q-dots by using nanoparticle tracking analysis (NTA) system.
The sensitivities of the cell origin markers for ECs (CD105, CD144) and EPCs (CD34, KDR) were evaluated.
The sensitivity and specificity were determined by using positive and negative markers for EXs (CD63), platelets (CD41), erythrocytes (CD235a), and microvesicles (Annexin V).
Moreover, the methods were further validated in particle-free plasma and patient samples.
Results showed that anti-CD105/anti-CD144 and anti-CD34/anti-KDR had the highest sensitivity and specificity for isolating and detecting EC-EXs and EPC-EXs, respectively.
The methods had the overall recovery rate of over 70% and were able to detect the dynamical changes of circulating EC-EXs and EPC-EXs in acute ischemic stroke.
In conclusion, we have developed sensitive and specific microbeads/Q-dots fluorescence NTA methods for EC-EX and EPC-EX isolation and detection, which will facilitate the functional study and biomarker discovery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Jinju& Guo, Runmin& Yang, Yi& Jacobs, Bradley& Chen, Su-Hong& Iwuchukwu, Ifeanyi…[et al.]. 2016. The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116273
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Jinju…[et al.]. The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116273
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Jinju& Guo, Runmin& Yang, Yi& Jacobs, Bradley& Chen, Su-Hong& Iwuchukwu, Ifeanyi…[et al.]. The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116273
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116273
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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