Improvement in Isolation and Identification of Mouse Oogonial Stem Cells
المؤلفون المشاركون
Lu, Zhiyong
Wu, Meng
Zhang, Jinjin
Xiong, Jiaqiang
Cheng, Jing
Shen, Wei
Luo, Aiyue
Fang, Li
Wang, Shixuan
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-09
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Female germline stem cells (FGSCs) or oogonial stem cells (OSCs) have the capacity to generate newborn oocytes and thus open a new door to fight ovarian aging and female infertility.
However, the production and identification of OSCs are difficult for investigators.
Rare amount of these cells in the ovary results in the failure of the acquisition of OSCs.
Furthermore, the oocyte formation by OSCs in vivo was usually confirmed using tissue sections by immunofluorescence or immunohistochemistry in previous studies.
STO or MEF feeder cells are derived from mouse, not human.
In our study, we modified the protocol.
The cells were digested from ovaries and cultured for 2-3 days and then were purified by magnetic-activated cell sorting (MACS).
The ovaries and fetus of mice injected with EGFP-positive OSCs were prepared and put on the slides to directly visualize oocyte and progeny formation under microscope.
Additionally, the human umbilical cord mesenchymal stem cells (hUC-MSCs) were also used as feeder cells to support the proliferation of OSCs.
The results showed that all the modified procedures can significantly improve and facilitate the generation and characterization of OSCs, and hUC-MSCs as feeder will be useful for isolation and proliferation of human OSCs avoiding contamination from mouse.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Zhiyong& Wu, Meng& Zhang, Jinjin& Xiong, Jiaqiang& Cheng, Jing& Shen, Wei…[et al.]. 2015. Improvement in Isolation and Identification of Mouse Oogonial Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116301
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Zhiyong…[et al.]. Improvement in Isolation and Identification of Mouse Oogonial Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116301
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Zhiyong& Wu, Meng& Zhang, Jinjin& Xiong, Jiaqiang& Cheng, Jing& Shen, Wei…[et al.]. Improvement in Isolation and Identification of Mouse Oogonial Stem Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116301
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116301
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر