Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface
المؤلفون المشاركون
Chen, Ying-Hui
Liu, Szu-Heng
Yu, Chen-Hsiang
Chang, Chiung-Hsin
Solis, Mairim Alexandra
Huang, Lynn L. H.
Wong, Tzyy Yue
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-29
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Our previous results showed that hyaluronan (HA) preserved human placenta-derived mesenchymal stem cells (PDMSC) in a slow cell cycling mode similar to quiescence, the pristine state of stem cells in vivo, and HA was found to prevent murine adipose-derived mesenchymal stem cells from senescence.
Here, stable isotope labeling by amino acid in cell culture (SILAC) proteomic profiling was used to evaluate the effects of HA on aging phenomenon in stem cells, comparing (1) old and young passage PDMSC cultured on normal tissue culture surface (TCS); (2) old passage on HA-coated surface (CHA) compared to TCS; (3) old and young passage on CHA.
The results indicated that senescence-associated protein transgelin (TAGLN) was upregulated in old TCS.
Protein CYR61, reportedly senescence-related, was downregulated in old CHA compared to old TCS.
The SIRT1-interacting Nicotinamide phosphoribosyltransferase (NAMPT) increased by 2.23-fold in old CHA compared to old TCS, and is 0.48-fold lower in old TCS compared to young TCS.
Results also indicated that components of endoplasmic reticulum associated degradation (ERAD) pathway were upregulated in old CHA compared to old TCS cells, potentially for overcoming stress to maintain cell function and suppress senescence.
Our data points to pathways that may be targeted by HA to maintain stem cells youth.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wong, Tzyy Yue& Chen, Ying-Hui& Liu, Szu-Heng& Solis, Mairim Alexandra& Yu, Chen-Hsiang& Chang, Chiung-Hsin…[et al.]. 2015. Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116308
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wong, Tzyy Yue…[et al.]. Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1116308
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wong, Tzyy Yue& Chen, Ying-Hui& Liu, Szu-Heng& Solis, Mairim Alexandra& Yu, Chen-Hsiang& Chang, Chiung-Hsin…[et al.]. Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1116308
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116308
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر