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Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord
المؤلفون المشاركون
Emnett, Ryan J.
Kaul, Aparna
Babic, Aleksandar
Geiler, Vicki
Regan, Donna
Gross, Gilad
Akel, Salem
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-21
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
Recent studies have demonstrated that the umbilical cord (UC) is an excellent source of mesenchymal stromal cells (MSCs).
However, current protocols for extracting and culturing UC-MSCs do not meet current good manufacturing practice (cGMP) standards, in part due to the use of xenogeneic reagents.
To support the development of a cGMP-compliant method, we have examined an enzyme-free isolation method utilizing tissue homogenization (t-H) followed by culture in human platelet lysate (PL) supplemented media.
The yield and viability of cells after t-H were comparable to those obtained after collagenase digestion (Col-D).
Importantly, kinetic analysis of cultured cells showed logarithmic growth over 10 tested passages, although the rate of cell division was lower for t-H as compared to Col-D.
This slower growth of t-H-derived cells was also reflected in their longer population doubling time.
Interestingly, there was no difference in the expression of mesenchymal markers and trilineage differentiation potential of cells generated using either method.
Finally, t-H-derived cells had greater clonogenic potential compared to Col-D/FBS but not Col-D/PL and were able to maintain CFU-F capacity through P7.
This bench scale study demonstrates the possibility of generating therapeutic doses of good quality UC-MSCs within a reasonable length of time using t-H and PL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Emnett, Ryan J.& Kaul, Aparna& Babic, Aleksandar& Geiler, Vicki& Regan, Donna& Gross, Gilad…[et al.]. 2016. Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116374
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Emnett, Ryan J.…[et al.]. Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116374
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Emnett, Ryan J.& Kaul, Aparna& Babic, Aleksandar& Geiler, Vicki& Regan, Donna& Gross, Gilad…[et al.]. Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116374
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116374
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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