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Targeted Inhibition of the miR-199a214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition
المؤلفون المشاركون
Luo, Yumei
Zhu, Detu
Wang, Shu
Xu, Xuehu
An, Xiuli
Sun, Xiaofang
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-14
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
The human induced pluripotent stem cell (hiPSC) provides a breakthrough approach that helps overcoming ethical and allergenic challenges posed in application of neural stem cells (NSCs) in targeted cancer gene therapy.
However, the tumor-tropic capacity of hiPSC-derived NSCs (hiPS-NSCs) still has much room to improve.
Here we attempted to promote the tumor tropism of hiPS-NSCs by manipulating the activity of endogenous miR-199a/214 cluster that is involved in regulation of hypoxia-stimulated cell migration.
We first developed a baculovirus-delivered CRISPR interference (CRISPRi) system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9).
We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster.
Meanwhile, the expression levels of their targets related to regulation of hypoxia-stimulated cell migration, such as HIF1A, MET, and MAPK1, were upregulated.
Further migration assays demonstrated that the targeted inhibition of the miR-199a/214 cluster significantly enhanced the tumor tropism of hiPS-NSCs both in vitro and in vivo.
These findings suggest a novel application of CRISPRi in NSC-based tumor-targeted gene therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Yumei& Xu, Xuehu& An, Xiuli& Sun, Xiaofang& Wang, Shu& Zhu, Detu. 2016. Targeted Inhibition of the miR-199a214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition. Stem Cells International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116405
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Yumei…[et al.]. Targeted Inhibition of the miR-199a214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1116405
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Yumei& Xu, Xuehu& An, Xiuli& Sun, Xiaofang& Wang, Shu& Zhu, Detu. Targeted Inhibition of the miR-199a214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1116405
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116405
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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