The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles

المؤلفون المشاركون

Yildirim, Selin
Zibandeh, Noushin
Genc, Deniz
Ozcan, Elif Merve
Goker, Kamil
Akkoc, Tunc

المصدر

Stem Cells International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-12-06

دولة النشر

مصر

عدد الصفحات

15

الملخص EN

Aim.

To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs).

Method.

Mesenchymal stem cells were isolated from three sources in the orofacial region.

Characterization and PCR analyses were performed.

Lymphocytes were isolated from healthy peripheral venous blood.

Lymphocytes were cocultured with stem cells in the presence and absence of IFN-γ and stimulated with anti-CD2, anti-CD3, and anti-CD28 for 3 days.

Then, lymphocyte proliferation, the number of CD4+FoxP3+ T regulatory cells, and the levels of Fas/Fas ligand, IL-4, IL-10, and IFN-γ in the culture supernatant were measured.

Results.

The DFSCs exhibited an enhanced differentiation capacity and an increased number of CD4+FoxP3+ T lymphocytes and suppressed the proliferation and apoptosis of PBMCs compared with SHEDs and DPSCs.

The addition of IFN-γ augmented the proliferation of DFSCs.

Furthermore, the DFSCs suppressed IL-4 and IFN-γ cytokine levels and enhanced IL-10 levels compared with the other cell sources.

Conclusion.

These results suggest that IFN-γ stimulates DFSCs by inducing an immunomodulatory effect on the PBMCs of healthy donors while suppressing apoptosis and proliferation and increasing the number of CD4+FoxP3+ cells.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yildirim, Selin& Zibandeh, Noushin& Genc, Deniz& Ozcan, Elif Merve& Goker, Kamil& Akkoc, Tunc. 2015. The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles. Stem Cells International،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1116608

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yildirim, Selin…[et al.]. The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1116608

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yildirim, Selin& Zibandeh, Noushin& Genc, Deniz& Ozcan, Elif Merve& Goker, Kamil& Akkoc, Tunc. The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1116608

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1116608