![](/images/graphics-bg.png)
Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation
المؤلفون المشاركون
Wright, Karina
Turner, Sarah A.
Jones, Philip N.
Balain, Birender
Roberts, Sally
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-10
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Much emphasis has been placed recently on the repair of degenerate discs using implanted cells, such as disc cells or bone marrow derived mesenchymal stem cells (MSCs).
This study examines the temporal response of bovine and human nucleus pulposus (NP) cells and MSCs cultured in monolayer following exposure to altered levels of glucose (0, 3.15, and 4.5 g/L) and foetal bovine serum (0, 10, and 20%) using an automated time-lapse imaging system.
NP cells were also exposed to the cell death inducers, hydrogen peroxide and staurosporine, in comparison to serum starvation.
We have demonstrated that human NP cells show an initial “shock” response to reduced nutrition (glucose).
However, as time progresses, NP cells supplemented with serum recover with minimal evidence of cell death.
Human NP cells show no evidence of proliferation in response to nutrient supplementation, whereas MSCs showed greater response to increased nutrition.
When specifically inducing NP cell death with hydrogen peroxide and staurosporine, as expected, the cell number declined.
These results support the concept that implanted NP cells or MSCs may be capable of survival in the nutrient-poor environment of the degenerate human disc, which has important clinical implications for the development of IVD cell therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Turner, Sarah A.& Wright, Karina& Jones, Philip N.& Balain, Birender& Roberts, Sally. 2016. Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116756
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Turner, Sarah A.…[et al.]. Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116756
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Turner, Sarah A.& Wright, Karina& Jones, Philip N.& Balain, Birender& Roberts, Sally. Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116756
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116756
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)