Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells
المؤلفون المشاركون
Slenders, E.
Paesen, Rik
Ameloot, Marcel
Donders, Raf
Sanen, Kathleen
Gyselaers, Wilfried
Stinissen, Piet
Hellings, Niels
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-26
دولة النشر
مصر
عدد الصفحات
15
الملخص EN
In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo.
Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap.
Both techniques enable the detection of cells and extracellular structures, based on intrinsic properties of the specific tissue and intracellular molecules under optical irradiation.
TPM imaging and SHG imaging have been used for label-free monitoring of stem cells differentiation, assessment of their behavior in biocompatible scaffolds, and even cell tracking in vivo.
In this study, we show that TPM and SHG can accurately depict the umbilical cord architecture and visualize individual cells both in situ and during culture initiation, without the use of exogenously applied labels.
In combination with nuclear DNA staining, we observed a variance in fluorescent intensity in the vessel walls.
In addition, antibody staining showed differences in Oct4, αSMA, vimentin, and ALDH1A1 expression in situ, indicating functional differences among the umbilical cord cell populations.
In future research, marker-free imaging can be of great added value to the current antigen-based staining methods for describing tissue structures and for the identification of progenitor cells in their tissue of origin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Donders, Raf& Sanen, Kathleen& Paesen, Rik& Slenders, E.& Gyselaers, Wilfried& Stinissen, Piet…[et al.]. 2016. Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1116761
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Donders, Raf…[et al.]. Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1116761
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Donders, Raf& Sanen, Kathleen& Paesen, Rik& Slenders, E.& Gyselaers, Wilfried& Stinissen, Piet…[et al.]. Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1116761
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116761
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر