Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model
المؤلفون المشاركون
Yakisich, Juan Sebastian
Azad, Neelam
Venkatadri, Rajkumar
Kulkarni, Yogesh
Wright, Clayton
Kaushik, Vivek
Iyer, Anand Krishnan V.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-06
دولة النشر
مصر
عدد الصفحات
6
الملخص EN
Like with most solid tumors, the presence of a subpopulation of cancer stem cells (CSCs) or cancer stem-like cells (CS-LCs) has been associated with chemoresistance and tumor relapse in lung cancer cells.
In the absence of serum, CSCs/CS-LCs have the ability to grow as lung tumorspheres (LTSs), and this system is routinely used for isolation and characterization of putative CSCs/CS-LCs.
Methods to isolate LTSs are usually performed in serum-free media supplemented with specific additives such as epidermal growth factor and basic fibroblast growth factor.
In this study, we report the generation of LTSs without the addition of any external mitogenic stimulation.
LTSs generated in this manner demonstrated several traits usually associated with increased stemness such as elevated expression of the stemness-associated marker Sox2 and increased chemoresistance to conventional anticancer drugs.
In addition, we report that the FDA-approved drug Digitoxin, at concentration close to its therapeutic level, decreased the viability of LTSs and downregulated Sox2 independent of the PI3K/AKT pathway.
The potential use of LTSs generated without the addition of any external mitogenic stimulation to study the role of specific factor(s) associated with stemness properties is also discussed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yakisich, Juan Sebastian& Azad, Neelam& Venkatadri, Rajkumar& Kulkarni, Yogesh& Wright, Clayton& Kaushik, Vivek…[et al.]. 2016. Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model. Stem Cells International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1116774
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yakisich, Juan Sebastian…[et al.]. Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1116774
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yakisich, Juan Sebastian& Azad, Neelam& Venkatadri, Rajkumar& Kulkarni, Yogesh& Wright, Clayton& Kaushik, Vivek…[et al.]. Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1116774
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116774
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر