A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells
المؤلفون المشاركون
Kleijn, A.
Kloezeman, J. J.
Balvers, R. K.
Kaaij, M. van der
Dirven, C. M. F.
Leenstra, S.
Lamfers, M. L. M.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-05
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Serum-free culture methods for patient-derived primary glioma cultures, selecting for glioma stem-like cells (GSCs), are becoming the gold standard in neurooncology research.
These GSCs can be implemented in drug screens to detect patient-specific responses, potentially bridging the translational gap to personalized medicine.
Since numerous compounds are available, a rapid and reliable readout for drug efficacies is required.
This can be done using approaches that measure viability, confluency, cytotoxicity, or apoptosis.
To determine which assay is best suitable for drug screening, 10 different assays were systematically tested on established glioma cell lines and validated on a panel of GSCs.
General applicability was assessed using distinct treatment modalities, being temozolomide, radiation, rapamycin, and the oncolytic adenovirus Delta24-RGD.
The apoptosis and cytotoxicity assays did not unequivocally detect responses and were excluded from further testing.
The NADH- and ATP-based viability assays revealed comparable readout for all treatments; however, the latter had smaller standard deviations and direct readout.
Importantly, drugs that interfere with cell metabolism require alternative techniques such as confluency monitoring to accurately measure treatment effects.
Taken together, our data suggest that the combination of ATP luminescence assays with confluency monitoring provides the most specific and reproducible readout for drug screening on primary GSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kleijn, A.& Kloezeman, J. J.& Balvers, R. K.& Kaaij, M. van der& Dirven, C. M. F.& Leenstra, S.…[et al.]. 2016. A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116776
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kleijn, A.…[et al.]. A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1116776
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kleijn, A.& Kloezeman, J. J.& Balvers, R. K.& Kaaij, M. van der& Dirven, C. M. F.& Leenstra, S.…[et al.]. A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1116776
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116776
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر