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p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation
المؤلفون المشاركون
Höfig, Ines
Ingawale, Yashodhara
Atkinson, Michael J.
Hertlein, Heidi
Nelson, Peter J.
Rosemann, Michael
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-16
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Mesenchymal stem cells (MSCs) are a source of adult multipotent cells important in tissue regeneration.
Murine MSCs are known to proliferate poorly in vitro under normoxia.
The aim of this study is to analyze the interaction of nonphysiological high oxygen and low-dose γ-irradiation onto growth, senescence, and DNA damage.
Tri-potent bone marrow-derived MSCs from p53 wildtype and p53−/− mice were cultured under either 21% or 2% O2.
Long-term observations revealed a decreasing ability of wildtype mMSCs to proliferate and form colonies under extended culture in normoxia.
This was accompanied by increased senescence under normoxia but not associated with telomere shortening.
After low-dose γ-irradiation, the normoxic wildtype cells further increased the level of senescence.
The number of radiation-induced γH2AX DNA repair foci was higher in mMSCs kept under normoxia but not in p53−/− cells.
P53-deficient MSCs additionally showed higher clonogeneity, lower senescence levels, and fewer γH2AX repair foci per cell as compared to their p53 wildtype counterparts irrespective of oxygen levels.
These results reveal that oxygen levels together with γ-irradiation and p53 status are interconnected factors modulating growth capacity of BM MSCs in long-term culture.
These efforts help to better understand and optimize handling of MSCs prior to their therapeutic use.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Höfig, Ines& Ingawale, Yashodhara& Atkinson, Michael J.& Hertlein, Heidi& Nelson, Peter J.& Rosemann, Michael. 2015. p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116925
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Höfig, Ines…[et al.]. p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116925
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Höfig, Ines& Ingawale, Yashodhara& Atkinson, Michael J.& Hertlein, Heidi& Nelson, Peter J.& Rosemann, Michael. p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116925
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116925
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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