Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation
المؤلفون المشاركون
Marrazzo, Pasquale
Paduano, Francesco
Palmieri, Francesca
Marrelli, Massimo
Tatullo, Marco
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-28
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Dental pulp is an accessible source of multipotent mesenchymal stromal cells (MSCs).
The perspective role of dental pulp stem cells (DPSCs) in regenerative medicine demands an in vitro expansion and in vivo delivery which must deal with the safety issues about animal serum, usually required in cell culture practice.
Human platelet lysate (PL) contains autologous growth factors and has been considered as valuable alternative to fetal bovine serum (FBS) in cell cultures.
The optimum concentration to be added of such supplement is highly dependent on its preparation whose variability limits comparability of results.
By in vitro experiments, we aimed to evaluate a standardised formulation of pooled PL.
A low selected concentration of PL (1%) was able to support the growth and maintain the viability of the DPSCs.
The use of PL in cell cultures did not impair cell surface signature typically expressed by MSCs and even upregulated the transcription of Sox2.
Interestingly, DPSCs cultured in presence of PL exhibited a higher healing rate after injury and are less susceptible to toxicity mediated by exogenous H2O2 than those cultured with FBS.
Moreover, PL addition was shown as a suitable option for protocols promoting osteogenic and chondrogenic differentiation of DPSCs.
Taken together, our results indicated that PL is a valid substitute of FBS to culture and differentiate DPSCs for clinical-grade use.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Marrazzo, Pasquale& Paduano, Francesco& Palmieri, Francesca& Marrelli, Massimo& Tatullo, Marco. 2016. Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117075
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Marrazzo, Pasquale…[et al.]. Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1117075
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Marrazzo, Pasquale& Paduano, Francesco& Palmieri, Francesca& Marrelli, Massimo& Tatullo, Marco. Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117075
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117075
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر