N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural StemProgenitor Cells Induced by Aβ 1–42 via the CB2 Receptor

المؤلفون المشاركون

Yin, Min
Wang, Zejian
Li, Wen-Qing
Liu, Sha
Hu, Yue
Lu, Yang

المصدر

Stem Cells International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-01-10

دولة النشر

مصر

عدد الصفحات

9

الملخص EN

Alzheimer’s disease, one of the neurodegenerative diseases, shows the progressive senescence of neural progenitor/stem cells.

N-Stearoyl-L-tyrosine (NsTyr) showed neuroprotective effect against chronic brain ischemia in previous reports.

In the present study, we find the antisenescent effects of NsTyr-2K in NSPCs induced by A β 1 – 42 in vitro.

Cell viability of NSPCs was evaluated by CCK8 assay; SA-β-gal staining was used to evaluate senescence of NSPCs.

CB receptors were detected by immunohistochemistry in NSPCs.

AM251 or AM630 was used to offset the anti-senescence effects afforded by NsTyr-2K.

The positive rate of SA-β-gal staining was significantly increased in NSPCs after incubation with A β 1 – 42 for 9 days.

CB receptors were found on the surface of NSPCs.

The expression level of CB1 receptors was significantly decreased in NSPCs after incubation with A β 1 – 42 .

This phenomenon was reversed dose-dependently by NsTyr-2K.

NsTyr-2K attenuated A β 1 – 42 induced NSPCs senescence dose-dependently, and its antisenescence effect was completely abolished by AM630.

A β 1 – 42 dose-dependently increased the prosenescence molecules p16 and Rb.

Their expression was inhibited by NsTyr-2K dose-dependently and blocked by AM630 in NSPCs.

These results suggest that NsTyr-2K can alleviate the senescence of NSPCs induced by A β 1 – 42 via CB2 receptor.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Li, Wen-Qing& Wang, Zejian& Liu, Sha& Hu, Yue& Yin, Min& Lu, Yang. 2016. N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural StemProgenitor Cells Induced by Aβ 1–42 via the CB2 Receptor. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117111

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Li, Wen-Qing…[et al.]. N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural StemProgenitor Cells Induced by Aβ 1–42 via the CB2 Receptor. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1117111

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Li, Wen-Qing& Wang, Zejian& Liu, Sha& Hu, Yue& Yin, Min& Lu, Yang. N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural StemProgenitor Cells Induced by Aβ 1–42 via the CB2 Receptor. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1117111

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1117111