Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children
المؤلفون المشاركون
Jung, Han-Sung
Kang, Chung-Min
Kim, Seong-Oh
Jeon, Mijeong
Choi, Hyung-Jun
Lee, Jae-Ho
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-30
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
The aim of this study was to compare the differential gene expression and stemness in the human gingiva and dental follicles (DFs) according to their biological characteristics.
Gingiva (n=9) and DFs (n=9) were collected from 18 children.
Comparative gene expression profiles were collected using cDNA microarray.
The expression of development, chemotaxis, mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSs) related genes was assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
Histological analysis was performed using hematoxylin-eosin and immunohistochemical staining.
Gingiva had greater expression of genes related to keratinization, ectodermal development, and chemotaxis whereas DFs exhibited higher expression levels of genes related to tooth and embryo development.
qRT-PCR analysis showed that the expression levels of iPSc factors including SOX2, KLF4, and C-MYC were 58.5±26.3, 12.4±3.5, and 12.2±1.9 times higher in gingiva and VCAM1 (CD146) and ALCAM (CD166) were 33.5±6.9 and 4.3±0.8 times higher in DFs.
Genes related to MSCs markers including CD13, CD34, CD73, CD90, and CD105 were expressed at higher levels in DFs.
The results of qRT-PCR and IHC staining supported the microarray analysis results.
Interestingly, this study demonstrated transcription factors of iPS cells were expressed at higher levels in the gingiva.
Given the minimal surgical discomfort and simple accessibility, gingiva is a good candidate stem cell source in regenerative dentistry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kang, Chung-Min& Kim, Seong-Oh& Jeon, Mijeong& Choi, Hyung-Jun& Jung, Han-Sung& Lee, Jae-Ho. 2016. Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117288
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kang, Chung-Min…[et al.]. Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1117288
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kang, Chung-Min& Kim, Seong-Oh& Jeon, Mijeong& Choi, Hyung-Jun& Jung, Han-Sung& Lee, Jae-Ho. Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1117288
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117288
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر