Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds
المؤلفون المشاركون
Abay, Nergis
Gurel Pekozer, Gorke
Ramazanoglu, Mustafa
Kose, Gamze Torun
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-23
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Designing and providing a scaffold are very important for the cells in tissue engineering.
Polybutylene succinate (PBS) has high potential as a scaffold for bone regeneration due to its capacity in cell proliferation and differentiation.
Also, stem cells from 3rd molar tooth germs were favoured in this study due to their developmentally and replicatively immature nature.
In this study, porcine dental germ stem cells (pDGSCs) seeded PBS scaffolds were used to investigate the effects of surface modification with fibronectin or laminin on these scaffolds to improve cell attachment, proliferation, and osteogenic differentiation for tissue engineering applications.
The osteogenic potentials of pDGSCs on these modified and unmodified foams were examined to heal bone defects and the effects of fibronectin or laminin modified PBS scaffolds on pDGSC differentiation into bone were compared for the first time.
For this study, MTS assay was used to assess the cytotoxic effects of modified and unmodified surfaces.
For the characterization of pDGSCs, flow cytometry analysis was carried out.
Besides, alkaline phosphatase (ALP) assay, von Kossa staining, real-time PCR, CM-Dil, and immunostaining were applied to analyze osteogenic potentials of pDGSCs.
The results of these studies demonstrated that pDGSCs were differentiated into osteogenic cells on fibronectin modified PBS foams better than those on unmodified and laminin modified PBS foams.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abay, Nergis& Gurel Pekozer, Gorke& Ramazanoglu, Mustafa& Kose, Gamze Torun. 2016. Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117330
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abay, Nergis…[et al.]. Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1117330
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abay, Nergis& Gurel Pekozer, Gorke& Ramazanoglu, Mustafa& Kose, Gamze Torun. Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117330
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117330
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر