BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia
المؤلفون المشاركون
Yang, Yongguang
Feng, Yanmin
Feng, Xue
Liao, Shangying
Wang, Xiuxia
Gan, Haiyun
Wang, Lixian
Lin, Xiwen
Han, Chunsheng
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-04
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Spermatogenesis is sustained by the proliferation and differentiation of spermatogonial stem cells (SSCs).
However, the molecules controlling these processes remain largely unknown.
Here, we developed a simplified high concentration serum-containing system for the culture of mouse SSCs.
Analysis of SSCs markers and transplantation results revealed that the cultured spermatogonia retained stem cell characteristics after long-term in vitro propagation.
Using this culture system, the expression and function of bone morphogenetic protein 4 (BMP4) were explored.
Immunostaining showed that BMP4 was predominantly expressed in germ cells and that its level increased as spermatogenesis progresses.
BMP4 receptors BMPR1A and BMPRII were present in spermatogonia, spermatocytes, and round spermatids.
Moreover, despite the mRNAs of these two genes being present in mouse Sertoli cells, only BMPRII was detected by using Western blotting assays.
While exogenous BMP4 by itself did not induce the expression of Stra8 and c-Kit, two marker genes of differentiating spermatogonia, a significant cooperative effect of BMP4 and retinoic acid (RA) was observed.
Moreover, pretreatment of cultured spermatogonia with the BMP4 antagonist Noggin could inhibit RA-induced expression of these two marker genes.
In conclusion, BMP4 may exert autocrine effects and act cooperatively with RA to induce the differentiation of spermatogonia in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Yongguang& Feng, Yanmin& Feng, Xue& Liao, Shangying& Wang, Xiuxia& Gan, Haiyun…[et al.]. 2016. BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia. Stem Cells International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117431
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Yongguang…[et al.]. BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1117431
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Yongguang& Feng, Yanmin& Feng, Xue& Liao, Shangying& Wang, Xiuxia& Gan, Haiyun…[et al.]. BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1117431
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117431
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر