Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice
المؤلفون المشاركون
Milano, Annalisa
Mazzetta, Francesca
Valente, Sabatino
Ranieri, Danilo
Leone, Laura
Botticelli, Andrea
Lauro, Salvatore
Torrisi, Maria Rosaria
Marchetti, Paolo
Onesti, Elisa Concetta
Raffa, S.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-27
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related mortality; nevertheless, there are few data regarding detection of circulating tumor cells (CTCs) in NSCLC, compared to other kinds of cancers in which their prognostic roles have already been defined.
This difference is likely due to detection methods based on the epithelial marker expression which ignore CTCs undergoing epithelial-mesenchymal transition (CTCsEMT).
Methods.
After optimization of the test with spiking experiments of A549 cells undergoing TGF-β1-induced EMT (A549EMT), the CTCsEMT were enriched by immunomagnetic depletion of leukocytes and then characterized by a RT-PCR assay based on the retrieval of epithelial and EMT-related genes.
Blood samples from ten metastatic NSCLC patients before starting treatment and during chemotherapy were used to test this approach by longitudinal monitoring.
Ten age- and sex-matched healthy subjects were also enrolled as controls.
Results.
Recovery experiments of spiked A549EMT cells showed that the RT-PCR assay is a reliable method for detection of CTCsEMT.
CTCsEMT were detected in three patients at baseline and in six patients after four cycles of cysplatin-based chemotherapy.
Longitudinal monitoring of three patients showed that the CTCsEMT detection is related to poor therapeutic response.
Conclusions.
The RT-PCR-based approach for the evaluation of CTCsEMT phenotype could be a promising and inexpensive tool to predict the prognosis and the therapeutic response in NSCLC patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Milano, Annalisa& Mazzetta, Francesca& Valente, Sabatino& Ranieri, Danilo& Leone, Laura& Botticelli, Andrea…[et al.]. 2018. Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice. Analytical Cellular Pathology،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1117448
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Milano, Annalisa…[et al.]. Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice. Analytical Cellular Pathology No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1117448
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Milano, Annalisa& Mazzetta, Francesca& Valente, Sabatino& Ranieri, Danilo& Leone, Laura& Botticelli, Andrea…[et al.]. Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice. Analytical Cellular Pathology. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1117448
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117448
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر