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Micropropagation, Micromorphological Studies, and In Vitro Flowering in Rungia pectinata L.
المؤلفون المشاركون
Shekhawat, Mahipal S.
Manokari, M.
Ravindran, C. P.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-08
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
A tissue culture protocol was developed for an important medicinal plant Rungia pectinata L.
in the present study.
Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2 solution.
Murashige and Skoog (MS) medium was used to establish the cultures of R.
pectinata.
The bud break was reported on MS medium supplemented with 1.0 mg L−1 6-benzylaminopurine (BAP).
About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded.
The shoots were further multiplied using MS medium augmented with 0.5 mg L−1 each of BAP and kinetin (Kin) + 0.1 mg L−1 indole-3 acetic acid (IAA).
Maximum 13.2 shoots per explant with 5.2 cm length were observed.
All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1 indole-3 butyric acid (IBA).
In vitro flowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod.
The plantlets were hardened in the greenhouse for two months and finally transferred to the field.
The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots under in vitro conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. 2016. Micropropagation, Micromorphological Studies, and In Vitro Flowering in Rungia pectinata L.. Scientifica،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117783
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shekhawat, Mahipal S.…[et al.]. Micropropagation, Micromorphological Studies, and In Vitro Flowering in Rungia pectinata L.. Scientifica No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1117783
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. Micropropagation, Micromorphological Studies, and In Vitro Flowering in Rungia pectinata L.. Scientifica. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1117783
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117783
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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