HPV E6E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides
المؤلفون المشاركون
Park, Kwang Hwa
Kim, Geehyuk
Taye, Jemberu
Yu, Kwangmin
Park, Sunyoung
Kim, Jungho
Kim, Sunghyun
Lee, Dongsup
Wang, Hye-young
Lee, Hyeyoung
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-14
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide.
Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis.
Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods.
Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted.
And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study.
However, it takes a long time to collect enough specimens to conduct statistical analysis.
Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted.
The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing.
And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Geehyuk& Taye, Jemberu& Yu, Kwangmin& Park, Sunyoung& Kim, Jungho& Kim, Sunghyun…[et al.]. 2019. HPV E6E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1117901
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Geehyuk…[et al.]. HPV E6E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides. Analytical Cellular Pathology No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1117901
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Geehyuk& Taye, Jemberu& Yu, Kwangmin& Park, Sunyoung& Kim, Jungho& Kim, Sunghyun…[et al.]. HPV E6E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1117901
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117901
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر