Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3KAktmTOR Survival Pathway
المؤلفون المشاركون
El-kott, Attalla Farag
Shati, Ali A.
Al-kahtani, Mohammed Ali
Alqahtani, Sultan
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-08
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
This study investigated the effect of acylated synthetic ghrelin (AG) on the survival and proliferation of human chemosensitive ovarian cancer cells (A2780) and explored some mechanisms of action with a focus on the p53 apoptotic pathway and PI3K/Akt and NF-κB survival pathways.
Human A2780 ovarian cancer cells were cultured with or without AG treatment in the presence or absence of cisplatin.
In some cases, cisplatin+AG-treated cells were pre-incubated either with [D-Lys3]-GHRP-6, a ghrelin receptor antagonist, or with LY294002, a PI3K inhibitor.
mRNA of ghrelin receptors(GHS-R1a and GHS-R1b), as well as, protein levels of GHS-R1a, were expressed abundantly in A2780 cells.
AG treatment did not affect the mRNA and protein levels of GHS-R1a and GHS-R1b in both control and Cis-treated cells.
However, while AG treatment had no effect on control cell viability, it significantly increased cell viability and proliferation and inhibited cell death in Cis-treated cells.
In both control and Cis-treated cells, AG treatment significantly increased PI3K/Akt/mTOR signaling and enhanced the nuclear accumulation of NF-κB.
Concomitantly, in both control and Cis-treated cells, AG significantly lowered the protein levels of p53, p-p53 (Ser16), PUMA, cytochrome C, and cleaved caspase-3.
Interestingly, pre-incubating the cells with either [D-Lys3]-GHRP-6 or LY294002 completely abolished the above-mentioned effect of AG in both control and Cis-treated cells.
In conclusion, the findings of this study show that AG promotes cell survival of the OC cells and renders them resistat to Cis therapy, an effect that is mediated by the activation of PI3K/Akt/mTOR and activation of NF-κB, and requires GHS-R1a.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El-kott, Attalla Farag& Shati, Ali A.& Al-kahtani, Mohammed Ali& Alqahtani, Sultan. 2019. Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3KAktmTOR Survival Pathway. Analytical Cellular Pathology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1117910
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El-kott, Attalla Farag…[et al.]. Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3KAktmTOR Survival Pathway. Analytical Cellular Pathology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1117910
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El-kott, Attalla Farag& Shati, Ali A.& Al-kahtani, Mohammed Ali& Alqahtani, Sultan. Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3KAktmTOR Survival Pathway. Analytical Cellular Pathology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1117910
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1117910
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر