Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALBc Mice
المؤلفون المشاركون
Do, Thi Thao
Do, Thi Phuong
Nguyen, Thi Nga
Nguyen, Thi Cuc
Vu, Thi Thu Phuong
Nguyen, Thi Giang An
المصدر
Advances in Materials Science and Engineering
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-02
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
Although L-Asparaginase (L-ASP) is an effective chemotherapeutic agent, it has side effects such as fever, skin rashes, chills, anaphylaxis, and severe allergic reactions.
Moreover, the short half-life of L-ASP reduces its antitumor activity.
To reduce its side effects and broaden its pharmaceutical applications, L-ASP obtained from Pectobacterium carotovorum was subjected to liposomal conjugation.
The enzyme was then loaded into liposomes using the hydrated thin-film method.
The in vitro cytotoxic activity of liposomal L-ASP was evaluated with the MTT assay using cancerous cell lines, and its antitumor effects were examined in Lewis lung carcinoma (LLC) tumorized mice.
The average size of the liposomes containing purified L-asparagine was 93.03 ± 0.49 nm.
They had a zeta potential of –15.45 ± 6.72 mV, polydispersity index of 0.22 ± 0.02, and encapsulation efficiency of 53.99 ± 5.44%.
The in vitro cytotoxic activity of liposomal L-ASP was less effective against LLC, MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), SK-LU-1 (human lung carcinoma), and NTERA-2 (pluripotent human embryonic carcinoma) cells than that of free L-ASP.
However, the antitumor activity of liposomal L-ASP was significantly greater than that of untrapped L-ASP at the same doses (6 UI/mouse) in terms of tumor size (6309.11 ± 414.06 mm3) and life span (35.00 ± 1.12 days).
This is the first time the antitumor activities of PEGylated nanoliposomal L-ASP have been assessed in LLC carcinoma tumor-induced BALB/c mice and showed significantly improved pharmacological properties compared to those of free L-ASP (P<0.05).
Thus, nanoliposomal L-ASP should be considered for its widening applications against carcinoma tumors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Do, Thi Thao& Do, Thi Phuong& Nguyen, Thi Nga& Nguyen, Thi Cuc& Vu, Thi Thu Phuong& Nguyen, Thi Giang An. 2019. Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALBc Mice. Advances in Materials Science and Engineering،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1119749
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Do, Thi Thao…[et al.]. Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALBc Mice. Advances in Materials Science and Engineering No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1119749
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Do, Thi Thao& Do, Thi Phuong& Nguyen, Thi Nga& Nguyen, Thi Cuc& Vu, Thi Thu Phuong& Nguyen, Thi Giang An. Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALBc Mice. Advances in Materials Science and Engineering. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1119749
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1119749
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر