Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1
المؤلفون المشاركون
Pfeiffer, D. U.
Thanasarasakulpong, Arunee
Poolperm, Pichayanut
Tangjitjaroen, Weerapongse
Varinrak, Thanya
Sawada, Takuo
Sthitmatee, Nattawooti
المصدر
Veterinary Medicine International
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-17
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity.
The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group.
Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P.
multocida strains.
Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P.
multocida strains.
The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups.
The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection.
In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group.
These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P.
multocida infection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Thanasarasakulpong, Arunee& Poolperm, Pichayanut& Tangjitjaroen, Weerapongse& Varinrak, Thanya& Sawada, Takuo& Pfeiffer, D. U.…[et al.]. 2016. Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1. Veterinary Medicine International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1120674
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Thanasarasakulpong, Arunee…[et al.]. Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1. Veterinary Medicine International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1120674
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Thanasarasakulpong, Arunee& Poolperm, Pichayanut& Tangjitjaroen, Weerapongse& Varinrak, Thanya& Sawada, Takuo& Pfeiffer, D. U.…[et al.]. Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1. Veterinary Medicine International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1120674
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1120674
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر