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Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119
المؤلفون المشاركون
Aslantas, Yaprak
Surmeli, Nur Basak
المصدر
Bioinorganic Chemistry and Applications
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-20
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Biocatalysts are sought-after in synthesis of pharmaceuticals and agrochemicals due to their high regioselectivity and enantioselectivity.
Among biocatalysts, heme-containing cytochrome P450 (P450) oxygenases are an attractive target since they catalyze oxidation of “unactivated” carbon-hydrogen bonds with high efficiency.
CYP119 is an acidothermophilic P450 from Sulfolobus acidocaldarius, which has the potential to be widely used as a biocatalyst since it shows activity at high temperatures and low pH.
Polyhistidine tags (His-tags) are widely used to simplify purification of proteins.
However, His-tags can cause changes to protein structure and function.
Here, we demonstrate the effects of His-tags on CYP119.
To this end, the His-tags were cloned at the N-terminus or C-terminus of the CYP119, and His-tagged proteins were expressed and isolated.
The thermostability and peroxidase activity of His-tagged CYP119s were tested and compared to wild type CYP119.
Results indicated that while addition of His-tags increased the yield and simplified isolation of CYP119, they also influenced the electronic structure of active site and the activity of the protein.
We show that N-terminal His-tagged CYP119 has desirable properties and potential to be used in industrial applications, but mechanistic studies using this protein need careful interpretation since the His-tag affects electronic properties of the active site heme iron.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Aslantas, Yaprak& Surmeli, Nur Basak. 2019. Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119. Bioinorganic Chemistry and Applications،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1122474
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Aslantas, Yaprak& Surmeli, Nur Basak. Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119. Bioinorganic Chemistry and Applications No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1122474
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Aslantas, Yaprak& Surmeli, Nur Basak. Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119. Bioinorganic Chemistry and Applications. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1122474
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1122474
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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