Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine
المؤلفون المشاركون
Zyad, Abdelmajid
El khalki, Lamyae
Tilaoui, Mounir
Jaafari, Abdeslam
Ait Mouse, Hassan
المصدر
Advances in Pharmacological and Pharmaceutical Sciences
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-01-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The present study attempts to investigate the cytotoxic activity of ethanol and ethyl acetate extracts of the Moroccan Berberis vulgaris and its major component berberine, together with exploring their antioxidant properties.
It also consists of studying the combination effect of berberine and S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, against the human breast adenocarcinoma cell line (MCF-7).
Using the MTT assay, we report a differential cytotoxic effect of ethanol and ethyl acetate extracts since the ethanol extract is more cytotoxic than the ethyl acetate one, with IC50 = 3.54 μg/mL and 596.71 μg/mL, respectively.
Interestingly, no cytotoxic effect was observed against normal cells.
Furthermore, these extracts showed a remarkable antioxidant activity as measured by the DPPH free radicals scavenging assay.
In fact, the IC50 values are 69.65 μg/mL and 77.75 μg/mL for the ethanol and ethyl acetate extracts, respectively.
In addition, several concentrations of berberine, when combined with the NO donor used at IC30, induced a synergistic cytotoxic activity at concentrations ranging from 8.40 μM to 33.60 μM, as revealed by the combination index values, using the Chou–Talalay method.
However, at the other concentrations tested, an antagonistic effect was observed.
The observed cytotoxicity was related to apoptosis induction as demonstrated by the annexin-V-streptavidin FITC-staining analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El khalki, Lamyae& Tilaoui, Mounir& Jaafari, Abdeslam& Ait Mouse, Hassan& Zyad, Abdelmajid. 2018. Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1122760
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El khalki, Lamyae…[et al.]. Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine. Advances in Pharmacological and Pharmaceutical Sciences No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1122760
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El khalki, Lamyae& Tilaoui, Mounir& Jaafari, Abdeslam& Ait Mouse, Hassan& Zyad, Abdelmajid. Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine. Advances in Pharmacological and Pharmaceutical Sciences. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1122760
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1122760
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر