Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348T
المؤلفون المشاركون
Rivero, Matías
Torres-Paris, Constanza
Muñoz, Rodrigo
Cabrera, Ricardo
Navarro, Claudio A.
Jerez, Carlos A.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-05
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Polyphosphates (PolyP) are linear polymers of orthophosphate residues that have been proposed to participate in metal resistance in bacteria and archaea.
In addition of having a CopA/CopB copper efflux system, the thermoacidophilic archaeon Metallosphaera sedula contains electron-dense PolyP-like granules and a putative exopolyphosphatase (PPXMsed, Msed_0891) and four presumed pho84-like phosphate transporters (Msed_0846, Msed_0866, Msed_1094, and Msed_1512) encoded in its genome.
In the present report, the existence of a possible PolyP-based copper-resistance mechanism in M.
sedula DSM 5348T was evaluated.
M.
sedula DSM 5348T accumulated high levels of phosphorous in the form of granules, and its growth was affected in the presence of 16 mM copper.
PolyP levels were highly reduced after the archaeon was subjected to an 8 mM CuSO4 shift.
PPXMsed was purified, and the enzyme was found to hydrolyze PolyP in vitro.
Essential residues for catalysis of PPXMsed were E111 and E113 as shown by a site-directed mutagenesis of the implied residues.
Furthermore, M.
sedula ppx, pho84-like, and copTMA genes were upregulated upon copper exposure, as determined by qRT-PCR analysis.
The results obtained support the existence of a PolyP-dependent copper-resistance system that may be of great importance in the adaptation of this thermoacidophilic archaeon to its harsh environment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rivero, Matías& Torres-Paris, Constanza& Muñoz, Rodrigo& Cabrera, Ricardo& Navarro, Claudio A.& Jerez, Carlos A.. 2018. Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348T. Archaea،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1122823
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rivero, Matías…[et al.]. Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348T. Archaea No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1122823
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rivero, Matías& Torres-Paris, Constanza& Muñoz, Rodrigo& Cabrera, Ricardo& Navarro, Claudio A.& Jerez, Carlos A.. Inorganic Polyphosphate, Exopolyphosphatase, and Pho84-Like Transporters May Be Involved in Copper Resistance in Metallosphaera sedula DSM 5348T. Archaea. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1122823
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1122823
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر