Upregulation of Immune Process-Associated Genes in RAW264.7 Macrophage Cells in Response to Burkholderia pseudomallei Infection
المؤلفون المشاركون
Du, Li
Pang, Feng
Wang, Fengyang
Li, Yaying
Li, Guohua
Peng, Dongmei
Cao, Ruiyong
Zhu, Shu
Yang, Xiaojian
Nie, Xin
Zhang, Zhenxing
Li, Baobao
Huang, Haifeng
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-04
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Melioidosis is a severe and fatal tropical zoonosis, which is triggered by Burkholderia pseudomallei.
To better understand the host’s response to infection of B.
pseudomallei, an RNA-Seq technology was used to confirm differentially expressed genes (DEGs) in RAW264.7 cells infected with B.
pseudomallei.
In total, 4668 DEGs were identified across three time points (4, 8, and 11 hours after infection).
Short Time-Series Expression Miner (STEM) analysis revealed the temporal gene expression profiles and identified seven significant patterns in a total of 26 profiles.
Kyoto Encyclopedia of Genes and Genomes (KEGG) was utilized to confirm significantly enriched immune process-associated pathways, and 10 DEGs, including Ccl9, Ifnb1, Tnfα, Ptgs2, Tnfaip3, Zbp1, Ccl5, Ifi202b, Nfkbia, and Nfkbie, were mapped to eight immune process-associated pathways.
Subsequent quantitative real-time PCR assays confirmed that the 10 DEGs were all upregulated during infection.
Overall, the results showed that B.
pseudomallei infection can initiate a time-series upregulation of immune process-associated DEGs in RAW264.7 macrophage cells.
The discovery of this article helps us better understand the biological function of the immune process-associated genes during B.
pseudomallei infection and may aid in the development of prophylaxis and treatment protocols for melioidosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peng, Dongmei& Pang, Feng& Cao, Ruiyong& Zhu, Shu& Yang, Xiaojian& Nie, Xin…[et al.]. 2018. Upregulation of Immune Process-Associated Genes in RAW264.7 Macrophage Cells in Response to Burkholderia pseudomallei Infection. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1124175
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peng, Dongmei…[et al.]. Upregulation of Immune Process-Associated Genes in RAW264.7 Macrophage Cells in Response to Burkholderia pseudomallei Infection. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1124175
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peng, Dongmei& Pang, Feng& Cao, Ruiyong& Zhu, Shu& Yang, Xiaojian& Nie, Xin…[et al.]. Upregulation of Immune Process-Associated Genes in RAW264.7 Macrophage Cells in Response to Burkholderia pseudomallei Infection. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1124175
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124175
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر