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PPIA, HPRT1, and YWHAZ Genes Are Suitable for Normalization of mRNA Expression in Long-Term Expanded Human Mesenchymal Stem Cells
المؤلفون المشاركون
Jeon, Ryoung-Hoon
Lee, Sung-Lim
Rho, Gyu-Jin
Lee, Won-Jae
Son, Young-Bum
Bharti, Dinesh
Shivakumar, Sharath Belame
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Long-term expansion of mesenchymal stem cells (MSCs) under defined culture conditions is necessary in human stem cell therapy.
However, it alters the characteristics of MSCs.
Since quantitative real time polymerase chain reaction (qRT-PCR) is widely used as one of the key analytical methods for comparative characterization, the validation of reference genes (RGs) for normalization under each experimental condition is important to achieve reliable qRT-PCR results.
Therefore, the most stable RGs for long-term expanded bone marrow- and umbilical cord blood-derived MSCs (BM-MSCs and UCB-MSCs) under defined culture conditions for up to 20 passages were evaluated.
The more apparent alterations in characteristics such as differentiation capacity, proliferation, senescence, and the expression of RGs were noted in BM-MSCs than UCB-MSCs during long-term expansion.
The RG validation programs (GeNorm and NormFinder) suggested that PPIA, HPRT1, and YWHAZ were suitable for normalization in qRT-PCR regardless of MSC types and long-term culture expansion, and the traditional RGs (ACTB and GAPDH) were less stable in long-term expanded MSCs.
In addition, the use of these RGs for normalization of OCT4 expression in long-term expanded BM-MSCs showed that a less stable RG (GAPDH) showed contrasting data compared to other RGs.
Therefore, the use of RGs such as PPIA, HPRT1, and YWHAZ for normalization in qRT-PCR experiments is highly recommended for long-term expanded MSCs to generate accurate and reliable data.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jeon, Ryoung-Hoon& Lee, Won-Jae& Son, Young-Bum& Bharti, Dinesh& Shivakumar, Sharath Belame& Lee, Sung-Lim…[et al.]. 2019. PPIA, HPRT1, and YWHAZ Genes Are Suitable for Normalization of mRNA Expression in Long-Term Expanded Human Mesenchymal Stem Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1124337
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jeon, Ryoung-Hoon…[et al.]. PPIA, HPRT1, and YWHAZ Genes Are Suitable for Normalization of mRNA Expression in Long-Term Expanded Human Mesenchymal Stem Cells. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1124337
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jeon, Ryoung-Hoon& Lee, Won-Jae& Son, Young-Bum& Bharti, Dinesh& Shivakumar, Sharath Belame& Lee, Sung-Lim…[et al.]. PPIA, HPRT1, and YWHAZ Genes Are Suitable for Normalization of mRNA Expression in Long-Term Expanded Human Mesenchymal Stem Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1124337
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124337
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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