Protective Effect of Astragaloside IV on Hepatic Injury Induced by Iron Overload
المؤلفون المشاركون
Xie, Dongyu
Zhou, Ping
Liu, Lin
Jiang, Wenjing
Xie, Haina
Zhang, Liang
Xie, Donghao
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-25
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Suitable content of iron is essential for human body, but iron overload is associated with many kinds of diseases including chronic liver damage.
Recently, researchers find that iron overload promotes hepatocyte autophagy and apoptosis.
However, the mechanism of iron overload in liver damage remains unclear.
In this study, Lo2 cells were selected as the research object, iron dextran was a model drug, and astragaloside IV was a therapeutic drug to explore the role of iron overload.
MTT assay and Annexin/PI double staining were used to measure cell viability and apoptosis.
Ultrastructure was observed by transmission electron microscopy.
The expression levels of apoptosis and autophagy-related proteins were determined by real-time PCR and Western Blot.
The results showed that iron dextran could significantly inhibit Lo2 cell viability and increase the apoptosis rate, while astragaloside IV could reverse the inhibition of Lo2 cell viability and decrease the apoptosis rate.
Transmission electron microscopy showed a significant increase in the number of autophagosomes after administration of iron dextran, and the application of astragaloside IV reduced the production of autophagosomes.
LC3II/I was significantly upregulated in the model group but decreased in the astragaloside IV treatment group, and P62 showed the opposite trend.
Iron dextran significantly upregulated the expression of Bax and downregulated Bcl2, while astragaloside IV reversed this trend.
Finally, the inhibition of hepcidin caused by iron dextran was counteracted by astragaloside IV.
In conclusion, the experimental results show that the iron overload model mainly induces excessive autophagy and apoptosis of hepatocytes, thus causing damage to hepatocytes, but astragaloside IV plays a certain therapeutic role in reversing this damage.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xie, Dongyu& Zhou, Ping& Liu, Lin& Jiang, Wenjing& Xie, Haina& Zhang, Liang…[et al.]. 2019. Protective Effect of Astragaloside IV on Hepatic Injury Induced by Iron Overload. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124348
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xie, Dongyu…[et al.]. Protective Effect of Astragaloside IV on Hepatic Injury Induced by Iron Overload. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1124348
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xie, Dongyu& Zhou, Ping& Liu, Lin& Jiang, Wenjing& Xie, Haina& Zhang, Liang…[et al.]. Protective Effect of Astragaloside IV on Hepatic Injury Induced by Iron Overload. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124348
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124348
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر