Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus
المؤلفون المشاركون
Zeng, Nanfang
Wang, Cong
Liu, Siyu
Miao, Qi
Han, Jun
Yang, Hanchun
Zhou, Lei
Guo, Xin
Ge, Xinna
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-23، 23ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-29
دولة النشر
مصر
عدد الصفحات
23
التخصصات الرئيسية
الملخص EN
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens and causes reproductive failure in sows and respiratory disease in growing pigs.
PRRSV mainly infects porcine alveolar macrophages (PAMs), leading to the subversion of innate and adaptive immunity of pigs.
The transcriptome analysis of gene expression profiles in PRRSV-infected PAMs is essential for understanding the pathogenesis of PRRSV.
Here we performed next-generation RNA sequencing and a comprehensive bioinformatics analysis to characterize the dynamic transcriptome landscapes in PAMs following PRRSV infection.
Totally 38222 annotated mRNAs, 12987 annotated long noncoding RNAs (lncRNAs), and 17624 novel lncRNAs in PRRSV-infected PAMs were identified through a transcripts computational identification pipeline.
The differentially expressed mRNAs and lncRNAs during PRRSV infection were characterized.
Several differentially expressed transcripts were validated using qRT-PCR.
Analyses on dynamic overrepresented GO terms and KEGG pathways in PRRSV-infected PAMs at different time points were performed.
Meanwhile the genes involved in IFN-related signaling pathways, proinflammatory cytokines and chemokines, phagocytosis, and antigen presentation and processing were significantly downregulated, indicating the aberrant function of PAMs during PRRSV infection.
Moreover, the differentially and highly expressed lncRNA XR_297549.1 was predicted to both cis-regulate and trans-regulate its neighboring gene, prostaglandin-endoperoxide synthase 2 (PTGS2), indicating its role in inflammatory response.
Our findings reveal the transcriptome profiles and differentially expressed mRNAs and lncRNAs in PRRSV-infected PAMs in vitro, providing valuable information for further exploration of PRRSV pathogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zeng, Nanfang& Wang, Cong& Liu, Siyu& Miao, Qi& Zhou, Lei& Ge, Xinna…[et al.]. 2018. Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International،Vol. 2018, no. 2018, pp.1-23.
https://search.emarefa.net/detail/BIM-1124442
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zeng, Nanfang…[et al.]. Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International No. 2018 (2018), pp.1-23.
https://search.emarefa.net/detail/BIM-1124442
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zeng, Nanfang& Wang, Cong& Liu, Siyu& Miao, Qi& Zhou, Lei& Ge, Xinna…[et al.]. Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-23.
https://search.emarefa.net/detail/BIM-1124442
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124442
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر