Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing
المؤلفون المشاركون
Kumburu, Happiness H.
Sonda, Tolbert
Leekitcharoenphon, Pimlapas
van Zwetselaar, Marco
Lukjancenko, Oksana
Alifrangis, Michael
Lund, Ole
Mmbaga, Blandina T.
Kibiki, Gibson
Aarestrup, Frank M.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-01-02
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Objective.
To determine molecular epidemiology of methicillin-resistant S.
aureus in Tanzania using whole genome sequencing.
Methods.
DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates.
Whole genome sequencing was performed on Illumina Miseq using paired-end 2×250 bp protocol.
Raw sequence data were analyzed using online tools.
Results.
Full susceptibility to vancomycin and chloramphenicol was observed.
Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested.
Multilocus sequence typing revealed 13 different sequence types among the 30 S.
aureus isolates, with ST-8 (n = seven, 23%) being the most common.
Gene detection in S.
aureus stains were as follows: mecA, 10 (33.3%); pvl, 5 (16.7%); tst, 2 (6.7%).
The SNP difference among the six Tanzanian ST-8 MRSA isolates ranged from 24 to 196 SNPs and from 16 to 446 SNPs when using the USA300_FPR3757 or the USA500_2395 as a reference, respectively.
The mutation rate was 1.38×10-11 SNPs/site/year or 1.4×10-6 SNPs/site/year as estimated by USA300_FPR3757 or the USA500_2395, respectively.
Conclusion.
S.
aureus isolates causing infections in hospitalized patients in Moshi are highly diverse and epidemiologically unrelated.
Temporal phylogenetic analysis provided better resolution on transmission and introduction of MRSA and it may be important to include this in future routines.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kumburu, Happiness H.& Sonda, Tolbert& Leekitcharoenphon, Pimlapas& van Zwetselaar, Marco& Lukjancenko, Oksana& Alifrangis, Michael…[et al.]. 2018. Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124890
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kumburu, Happiness H.…[et al.]. Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1124890
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kumburu, Happiness H.& Sonda, Tolbert& Leekitcharoenphon, Pimlapas& van Zwetselaar, Marco& Lukjancenko, Oksana& Alifrangis, Michael…[et al.]. Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124890
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124890
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر