MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes
المؤلفون المشاركون
Duan, Zhi-xi
Huang, Peng
Tu, Chao
Liu, Qing
Li, Shuang-qing
Long, Ze-ling
Li, Zhi-hong
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-05
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background and Aims.
A growing body of research has demonstrated that the degeneration of chondrocytes is the primary cause of osteoarthritis (OA).
Parathyroid hormone-related protein (PTHrP) can alleviate the degeneration of chondrocytes via promotion of chondrocyte proliferation and inhibition of terminal differentiation, but the underlying mechanism remains unknown.
This study aimed to identify the microRNAs (miRNAs) that may target PTHrP and regulate the proliferation and terminal differentiation of chondrocytes.
Methods.
Bioinformatic analysis was used to predict which miRNAs target PTHrP.
We collected human knee cartilage specimens to acquire the primary chondrocytes, which we then used to test the expression and function of the targeted miRNAs.
To explore the effects of miR-15a-5p on the putative binding sites, specific mimics or inhibitors were transfected into the chondrocytes.
Furthermore, a dual-luciferase reporter gene assay and chondrocyte degeneration-related factors were used to verify the possible mechanism.
Results.
The expression of PTHrP was upregulated in the OA chondrocytes, whilst miR-15a-5p was downregulated in the OA chondrocytes.
A negative correlation was observed between PTHrP and miR-15a-5p.
The knockdown of miR-15a-5p promoted the growth of chondrocytes and inhibited calcium deposition, whilst overexpression of miR-15a-5p reversed this trend.
The effect of miR-15a-5p overexpression was neutralised by PTHrP.
Dual-luciferase reporter assays revealed that PTHrP can be used as a novel targeting molecule for miR-15a-5p.
Conclusions.
miR-15a-5p promotes the degeneration of chondrocytes by targeting PTHrP and, in addition to helping us understand the development of OA, may be a potential biomarker of OA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duan, Zhi-xi& Huang, Peng& Tu, Chao& Liu, Qing& Li, Shuang-qing& Long, Ze-ling…[et al.]. 2019. MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1124943
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duan, Zhi-xi…[et al.]. MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1124943
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duan, Zhi-xi& Huang, Peng& Tu, Chao& Liu, Qing& Li, Shuang-qing& Long, Ze-ling…[et al.]. MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1124943
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1124943
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر