MiR-29a Inhibits Glioma Tumorigenesis through a Negative Feedback Loop of TRAF4Akt Signaling
المؤلفون المشاركون
Liu, Yongjie
Duan, Naiquan
Duan, Shibo
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-14
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
MiR-29a is known as a repressor of human cancer.
However, its relevance in glioma proliferation and invasion remains largely unknown.
In this study, we aimed to investigate the function and mechanism of miR-29a in glioma tumorigenesis.
Methods.
The expression of miR-29a was determined by using qRT-PCR.
CCK-8, wound healing, and transwell invasion assays were carried out to analyze the effects of miR-29a in glioblastoma cells.
qRT-PCR, luciferase reporter, and western blot experiments were done to validate the targeting of TRAF4/Akt pathway by miR-29a.
The expression correlation between levels of TRAF4 and miR-29a was analyzed.
Regulation of miR-29a expression by enhanced/reduced TRAF4/Akt expression was finally confirmed by qRT-PCR.
Results.
MiR-29a was decreased in the glioma tissues, especially in those at higher grades.
Following its mimic transfection, we validated that miR-29a inhibited cell proliferation, migration, and invasion.
Consistently, miR-29a inhibition induced the opposite effects on cell proliferation, migration, and invasion.
We confirmed TRAF4 as a direct target of miR-29a, which might mediate the Akt pathway activation.
We showed a significantly negative expression correlation between TRAF4 and miR-29a in normal and glioma tissues.
Finally we observed an upregulation of miR-29a in TRAF4/Akt activated cells.
Conclusion.
MiR-29a is critical tumor suppressor for glioma tumorigenesis by forming a negative feedback loop of TRAF4/Akt signaling and represents a potent therapeutic candidate for treating gliomas.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yongjie& Duan, Naiquan& Duan, Shibo. 2018. MiR-29a Inhibits Glioma Tumorigenesis through a Negative Feedback Loop of TRAF4Akt Signaling. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125067
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yongjie…[et al.]. MiR-29a Inhibits Glioma Tumorigenesis through a Negative Feedback Loop of TRAF4Akt Signaling. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125067
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yongjie& Duan, Naiquan& Duan, Shibo. MiR-29a Inhibits Glioma Tumorigenesis through a Negative Feedback Loop of TRAF4Akt Signaling. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125067
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125067
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر