Modulation of Corneal FAKPI3KAkt Signaling Expression and of Metalloproteinase-2 and Metalloproteinase-9 during the Development of Herpes Simplex Keratitis
المؤلفون المشاركون
Wang, Yu-jing
Yang, Yan-ning
Ke, Lan
Li, Jing wei
Wang, Bo
Yang, Wanju
Yan, Jiangbo
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
To observe the expression of MMP-2 and MMP-9 and of the FAK/PI3K/Akt signaling pathway in HSK.
Fifty BALB/c mice were infected to establish the model and killed on days 0, 2, 7, 14, and 28.
The cornea samples were prepared, respectively.
Slit lamp examination, immunofluorescence staining, reverse transcription PCR, and Western blot were used to detect the index.
After HSV-1 infection, different degrees of epithelial or stromal damage and corneal opacity were observed.
Immunofluorescence staining showed that the expressions of MMP-2 and MMP-9 at different levels of corneal tissue were observed on the 0d, 2d, 7d, 14d, and 28d.
Compared with 0d, the relative expression levels of MMP-2 and MMP-9 mRNA at 2d, 7d, 14d, and 28d were significantly increased (all P< 0.05).
Compared with 14d, the relative expression of MMP-2 and MMP-9 mRNA decreased on the 2d, 7d, and 28d (all P< 0.05).
Western blot showed that the protein expressions of p-FAK, p-PI3K, p-Akt, MMP-2, and MMP-9 at 2d, 14d, and 28d were all significantly higher than 0d (all P< 0.05).
At 14 d, the expressions of p-FAK, p-PI3K, p-Akt, and MMP-2 were significantly higher than those at 2d, 7d, and 28d (all P< 0.05).
The protein expression of FAK, PI3K, and Akt in corneal of mice in each time period had no significant (all P> 0.05).
These data suggest that FAK/PI3K/Akt signaling pathway and MMP-2 and MMP-9 may be involved in the development of HSK.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ke, Lan& Yang, Yan-ning& Li, Jing wei& Wang, Bo& Wang, Yu-jing& Yang, Wanju…[et al.]. 2019. Modulation of Corneal FAKPI3KAkt Signaling Expression and of Metalloproteinase-2 and Metalloproteinase-9 during the Development of Herpes Simplex Keratitis. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125164
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ke, Lan…[et al.]. Modulation of Corneal FAKPI3KAkt Signaling Expression and of Metalloproteinase-2 and Metalloproteinase-9 during the Development of Herpes Simplex Keratitis. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125164
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ke, Lan& Yang, Yan-ning& Li, Jing wei& Wang, Bo& Wang, Yu-jing& Yang, Wanju…[et al.]. Modulation of Corneal FAKPI3KAkt Signaling Expression and of Metalloproteinase-2 and Metalloproteinase-9 during the Development of Herpes Simplex Keratitis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125164
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125164
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر