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Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico
المؤلفون المشاركون
García-Flores, María Martha
Pérez, J. L. Tórtora
Mendiola, Wolfang P. S.
Martínez, Humberto A.
Cuevas-Romero, Sandra
Cerriteño, José L.
Ramírez, Hugo
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Small ruminant lentiviruses (SRLVs) belong to the genus Lentivirus in the Retroviridae family.
There are five genotypes (A, B, C, D, and E), where genotypes A and B have a global distribution and genotypes C, D, and E are limited to Europe.
The presence of SRLV has been confirmed in Mexico, with genotype B detected in the central region of the country.
We examined the presence of SRLVs and genotype prevalence in 1014 sheep and 1383 goats from 12 Mexican states.
Using a commercial competitive ELISA (cELISA) test, we detected SRLV antibodies in 107 sheep (10.55%) and 466 goats (33.69%).
We used an endpoint PCR to amplify the LTR region on seropositive animals.
A total of 50 sheep and 75 goats tested positive via PCR.
Positive amplicons from 11 sheep and 17 goats from ten Mexican States were cloned and sequenced.
With the LTR sequence data obtained in this study, a phylogenetic analysis was performed; we also constructed a phylogenetic tree using the obtained sequences and GenBank’s available sequences.
All studied sequences were associated with genotype B, specifically with the FESC-752 isolate previously identified in Mexico.
Highly conserved transcription factor binding sites were observed in analyzed alignments, such as AML (vis), AP-4, and TATA box.
However, we identified nucleotide differences at site AP-1 that suggest function loss.
Our study found that ovine and caprine genotype B SRLVs are widely distributed in Mexico; a highly conserved LTR region among the sequences evaluated in this study was also found.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mendiola, Wolfang P. S.& Pérez, J. L. Tórtora& Martínez, Humberto A.& García-Flores, María Martha& Cuevas-Romero, Sandra& Cerriteño, José L.…[et al.]. 2019. Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125239
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mendiola, Wolfang P. S.…[et al.]. Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125239
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mendiola, Wolfang P. S.& Pérez, J. L. Tórtora& Martínez, Humberto A.& García-Flores, María Martha& Cuevas-Romero, Sandra& Cerriteño, José L.…[et al.]. Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125239
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125239
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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