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Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication
المؤلفون المشاركون
Wang, Yuchen
Chen, Xiangdong
Chen, Beibei
Sima, Linshan
Cao, Mengzhuo
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-01
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Haloarchaeon Natrinema sp.
J7, the first reported archaeon harboring both plasmid and chromosome-based temperate viruses, is a useful model for investigating archaeal virus-host and virus-virus interactions.
However, the lack of genetic tools has limited such studies.
On the basis of the automatically replicating sequences of the J7 chromosome and the pyrF marker, we constructed seven vectors, six of which were confirmed to possess replication ability in a pyrF-deletion derivative of J7 (J7-F).
Among these vectors, pFJ1, pFJ4, and pFJ6 could be transformed into the host strain with relatively high efficiency (approximately 103 colony-forming units/μg DNA) and were present at about one copy per chromosome.
These three vectors could be stably maintained in J7-F without selection and were used for heterologous protein expression.
Only pFJ6 was found to be present in the transformed cells in an exclusively episomal, nonintegrated state (one copy per chromosome).
In contrast, some pFJ1 and pFJ4 DNA was probably integrated into the J7-F chromosome.
In addition, pFJ6 was found to be compatible with pYCJ in J7 cells, suggesting that these two vectors could be used for further studies of virus-virus and virus-host interactions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yuchen& Chen, Beibei& Sima, Linshan& Cao, Mengzhuo& Chen, Xiangdong. 2017. Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication. Archaea،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1125454
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yuchen…[et al.]. Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication. Archaea No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1125454
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yuchen& Chen, Beibei& Sima, Linshan& Cao, Mengzhuo& Chen, Xiangdong. Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication. Archaea. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1125454
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125454
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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