Prevalence, Genetic Heterogeneity, and Antibiotic Resistance Profile of Listeria spp. and Listeria monocytogenes at Farm Level: A Highlight of ERIC- and BOX-PCR to Reveal Genetic Diversity
المؤلفون المشاركون
Kasing, A.
Maurice Bilung, Lesley
Sin Chai, Lai
Tahar, Ahmad Syatir
Ted, Chong Kian
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-03
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
This study aimed to identify Listeria spp.
and L.
monocytogenes, characterize the isolates, and determine the antibiotic resistance profiles of the isolates Listeria spp.
and L.
monocytogenes in fresh produce, fertilizer, and environmental samples from vegetable farms (organic and conventional farms).
A total of 386 samples (vegetables, soil, water, and fertilizer with manure) were examined.
The identification of bacterial isolates was performed using PCR and characterized using ERIC-PCR and BOX-PCR.
The discriminating power of the typing method was analyzed using Simpson’s Index of Diversity.
Thirty-four (n=34) Listeria isolates were subjected to antimicrobial susceptibility test using the disc-diffusion technique.
The PCR analysis revealed that Listeria spp.
were present in 7.51% (29/386) of all the samples (vegetable, soil, fertilizer, and water).
None of the samples examined were positive for the presence of L.
monocytogenes.
Percentages of 100% (15/15) and 73.30% (11/15) of the Listeria spp.
isolated from vegetables, fertilizer, and soil from organic farm B had indistinguishable DNA fingerprints by using ERIC-PCR and BOX-PCR, respectively.
Listeria spp.
isolated from 86 samples of vegetable, fertilizer, and environment of organic farm A and conventional farm C had distinct DNA fingerprints.
Simpson’s Index of Diversity, D, of ERIC-PCR and BOX-PCR is 0.604 and 0.888, respectively.
Antibiotic susceptibility test revealed that most of the Listeria spp.
in this study were found to be resistant to ampicillin, rifampin, penicillin G, tetracycline, clindamycin, cephalothin, and ceftriaxone.
The isolates had MAR index ranging between 0.31 and 0.85.
In conclusion, hygienic measures at farm level are crucial to the reduction of Listeria transmission along the food chain.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maurice Bilung, Lesley& Sin Chai, Lai& Tahar, Ahmad Syatir& Ted, Chong Kian& Kasing, A.. 2018. Prevalence, Genetic Heterogeneity, and Antibiotic Resistance Profile of Listeria spp. and Listeria monocytogenes at Farm Level: A Highlight of ERIC- and BOX-PCR to Reveal Genetic Diversity. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1125605
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maurice Bilung, Lesley…[et al.]. Prevalence, Genetic Heterogeneity, and Antibiotic Resistance Profile of Listeria spp. and Listeria monocytogenes at Farm Level: A Highlight of ERIC- and BOX-PCR to Reveal Genetic Diversity. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1125605
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maurice Bilung, Lesley& Sin Chai, Lai& Tahar, Ahmad Syatir& Ted, Chong Kian& Kasing, A.. Prevalence, Genetic Heterogeneity, and Antibiotic Resistance Profile of Listeria spp. and Listeria monocytogenes at Farm Level: A Highlight of ERIC- and BOX-PCR to Reveal Genetic Diversity. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1125605
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125605
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر