Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects
المؤلفون المشاركون
Martin, W. Blake
Sicard, Renaud
Namin, Shabnam M.
Ganey, Timothy
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-16
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Debridement of the bone surface during a surgical fusion procedure initiates an injury response promoting a healing cascade of molecular mediators released over time.
Autologous grafts offer natural scaffolding to fill the bone void and to provide local bone cells.
Commercial bone grafting products such as allografts, synthetic bone mineral products, etc., are used to supplement or to replace autologous grafts by supporting osteoinductivity, osteoconductivity, and osteogenesis at the surgical site.
To assure osteogenic potential, preservation of allogeneic cells with cryoprotectants has been developed to allow for long-term storage and thus delivery of viable bone cells to the surgical site.
Dimethyl sulfoxide (DMSO) is an intracellular cryoprotectant commonly used because it provides good viability of the cells post-thaw.
However, there is known cytotoxicity reported for DMSO when cells are stored above cryogenic temperatures.
For most cellular bone graft products, the cryoprotectant is incorporated with the cells into the other mineralized bone and demineralized bone components.
During thawing, the DMSO may not be sufficiently removed from allograft products compared to its use in a cell suspension where removal by washing and centrifugation is available.
Therefore, both the allogeneic cell types in the bone grafting product and the local cell types at the bone grafting site could be affected as cytotoxicity varies by cell type and by DMSO content according to reported studies.
Overcoming cytotoxicity may be an additional challenge in the formation of bone at a wound or surgical site.
Other extracellular cryoprotectants have been explored as alternatives to DMSO which preserve without entering the cell membrane, thereby providing good cellular viability post-thaw and might abrogate the cytotoxicity concerns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martin, W. Blake& Sicard, Renaud& Namin, Shabnam M.& Ganey, Timothy. 2019. Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1125760
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martin, W. Blake…[et al.]. Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1125760
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martin, W. Blake& Sicard, Renaud& Namin, Shabnam M.& Ganey, Timothy. Methods of Cryoprotectant Preservation: Allogeneic Cellular Bone Grafts and Potential Effects. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1125760
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125760
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر