The Role of miRNA-132 against Apoptosis and Oxidative Stress in Heart Failure
المؤلفون المشاركون
Liu, Xuelei
Tong, Zhou
Chen, Keyan
Hu, Xiaofang
Jin, Hongxu
Hou, Mingxiao
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-25
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
To explore the effect of microRNA-132 of heart failure and provide theoretical guidance for clinical treatment of heart failure (HF).
Methods.
Peripheral blood was collected from HF patients.
RT-qPCR was used to determine microRNA-132 expression.
Mouse models of heart failure were established.
Color Doppler ultrasound was utilized to measure the changes of cardiac function.
HE and Masson staining were applied to observe pathological changes of the myocardium.
After H9C2 cells were transfected with microRNA-132, MTT assay was employed to detect the stability of H9C2 cells.
ELISA was used to measure the levels of oxidative stress factors.
Western blot assay and RT-qPCR were utilized to determine the expression of Bax, Bcl-2, TGF-β1, and smad3.
Results.
MicroRNA-132 expression was downregulated in HF patients’ blood.
After establishing mouse models of HF, cardiac function obviously decreased.
HE staining revealed the obvious edema and hypertrophy of cardiomyocytes.
Masson staining demonstrated that cardiomyocytes were markedly fibrotic.
After microRNA-132 transfection and H9C2 cell apoptosis induced by H2O2, antioxidant stress and antiapoptotic ability of the H9C2 cells obviously increased.
TGF-β1 and smad3 expression remarkably diminished.
Conclusion.
Overexpression of microRNA-132 dramatically increased the antioxidant stress and antiapoptotic ability of H9C2 cells and decreased the expression of TGF-β1 and smad3.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Xuelei& Tong, Zhou& Chen, Keyan& Hu, Xiaofang& Jin, Hongxu& Hou, Mingxiao. 2018. The Role of miRNA-132 against Apoptosis and Oxidative Stress in Heart Failure. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125819
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Xuelei…[et al.]. The Role of miRNA-132 against Apoptosis and Oxidative Stress in Heart Failure. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125819
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Xuelei& Tong, Zhou& Chen, Keyan& Hu, Xiaofang& Jin, Hongxu& Hou, Mingxiao. The Role of miRNA-132 against Apoptosis and Oxidative Stress in Heart Failure. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125819
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125819
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر