Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHACPLGAs Scaffold on Osteogenesis
المؤلفون المشاركون
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-24
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Tissue engineering bones take great advantages in massive bone defect repairing; under the induction of growth factors, seed cells differentiate into osteoblasts, and the scaffold materials gradually degrade and are replaced with neogenetic bones, which simulates the actual pathophysiological process of bone regeneration.
However, mechanism research is required and further developed to instruct elements selection and optimization.
In the present study, we prepared vascular endothelial growth factor/bone morphogenetic protein-2- nanohydroxyapatite/collagen (VEGF/ BMP-2- nHAC/ PLGAs) scaffolds and inoculated mouse MC3T3-E1 preosteoblasts to detect osteogenic indexes and activation of related signaling pathways.
The hypothesis is to create a three-dimensional environment that simulates bone defect repairing, and p38 mitogen-activated kinase (p38) inhibitor was applied and osterix shRNA was transferred into mouse MC3T3-E1 preosteoblasts to further investigate the molecular mechanism of crosstalk between BMP-2 and VEGF.
Our results demonstrated the following: (1) BMP-2 and VEGF were sustainably released from PLGAs microspheres.
(2) nHAC/PLGAs scaffold occupied a three-dimensional porous structure and has excellent physical properties.
(3) MC3T3-E1 cells proliferated and differentiated well in the scaffold.
(4) Osteogenic differentiation related factors expression of VEGF/BMP-2 loaded scaffold was obviously higher than that of other groups; p38 inhibitor SB203580 decreased the nucleus/cytoplasm ratio of osterix expression.
To conclude, the active artificial bone we prepared could provide a favorable growth space for MC3T3-E1 cells, and osteogenesis and maturation reinforced by simultaneous VEGF and BMP-2 treatment may be mainly through the activation of the p38 MAPK pathway to promote nuclear translocation of osterix protein.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Ting& Guo, Shu& Zhang, Hua. 2018. Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHACPLGAs Scaffold on Osteogenesis. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1125871
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Ting…[et al.]. Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHACPLGAs Scaffold on Osteogenesis. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1125871
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Ting& Guo, Shu& Zhang, Hua. Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHACPLGAs Scaffold on Osteogenesis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1125871
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125871
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر