The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation
المؤلفون المشاركون
Martens, S.
Freundt, Johanna K.
Frommeyer, Gerrit
Wötzel, Fabian
Huge, Andreas
Hoffmeier, Andreas
Eckardt, Lars
Lange, Philipp S.
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-29
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Introduction.
Cardiomyocyte remodelling in atrial fibrillation (AF) has been associated with both oxidative stress and endoplasmic reticulum (ER) stress and is accompanied by a complex transcriptional regulation.
Here, we investigated the role the oxidative stress and ER stress responsive bZIP transcription factor ATF4 plays in atrial cardiomyocyte viability and AF induced gene expression.
Methods.
HL-1 cardiomyocytes were subjected to rapid field stimulation.
Forced expression of ATF4 was achieved by adenoviral gene transfer.
Using global gene expression analysis and chromatin immunoprecipitation, ATF4 dependent transcriptional regulation was studied, and tissue specimen of AF patients was analysed by immunohistochemistry.
Results.
Oxidative stress and ER stress caused a significant reduction in cardiomyocyte viability and were associated with an induction of ATF4.
Accordingly, ATF4 was also induced by rapid field stimulation mimicking AF.
Forced expression of wild type ATF4 promoted cardiomyocyte death.
ATF4 was demonstrated to bind to the promoters of several cell stress genes and to induce the expression of a number of ATF4 dependent stress responsive genes.
Moreover, immunohistochemical analyses showed that ATF4 is expressed in the nuclei of cardiomyocytes of tissue specimen obtained from AF patients.
Conclusion.
ATF4 is expressed in human atrial cardiomyocytes and is induced in response to different types of cell stress.
High rate electrical field stimulation seems to result in ATF4 induction, and forced expression of ATF4 reduces cardiomyocyte viability.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Freundt, Johanna K.& Frommeyer, Gerrit& Wötzel, Fabian& Huge, Andreas& Hoffmeier, Andreas& Martens, S.…[et al.]. 2018. The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation. BioMed Research International،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1126012
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Freundt, Johanna K.…[et al.]. The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation. BioMed Research International No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1126012
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Freundt, Johanna K.& Frommeyer, Gerrit& Wötzel, Fabian& Huge, Andreas& Hoffmeier, Andreas& Martens, S.…[et al.]. The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1126012
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126012
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر