Chlorogenic Acid Attenuates Kidney IschemicReperfusion Injury via Reducing Inflammation, Tubular Injury, and Myofibroblast Formation
المؤلفون المشاركون
Arfian, Nur
Wahyudi, Danny A. P.
Zulfatina, Ingesti B.
Citta, Arsitya N.
Anggorowati, Nungki
Multazam, Ali
Romi, Muhammad M.
Sari, Dwi C. R.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-22
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Kidney ischemic/reperfusion (I/R) injury is the main cause of acute kidney injury (AKI) involving renal function deterioration, renal architecture damage, and inflammation.
This condition may lead to kidney fibrosis with epithelial to mesenchymal transition (EMT) and myofibroblast formation.
Inhibition of chronic effects of kidney I/R injury may provide effective strategies for treating AKI and chronic kidney diseases (CKDs).
Chlorogenic acid (CGA) is recognized as a powerful antioxidant, with anti-inflammatory and antifibrotic properties in many conditions.
However, the effect of CGA on kidney I/R injury has not been elucidated yet.
Kidney I/R injury was performed on male Swiss background mice (I/R group, n = 5, 3-4 months, 30–40 g) which underwent bilateral renal pedicles clamping for 30 minutes and then were euthanized on day three after operation.
Three groups of I/R were treated with 3 different doses of CGA intraperitoneally for 2 days: 3.5 (I/R + CGA1 group), 7 (I/R + CGA2 group), and 14 (I/R + CGA3 group) mg/kg of body weight.
Tubular injury was quantified based on Periodic Acid-Schiff staining, while reverse transcriptase PCR (RT-PCR) was performed to quantify mRNA expression of TGF-β1, vimentin, SOD-1, TLR-4, TNF-α, NF-κB and MCP-1.
Immunohistochemical staining was done to quantify proliferating cell nuclear antigen (PCNA), myofibroblast (α-SMA), SOD-1 and macrophage (CD68) number.
Kidney I/R demonstrated tubular injury and increased inflammatory mediator expression, macrophage number, and myofibroblast expansion.
Meanwhile, histological analysis showed lower tubular injury with higher epithelial cell proliferation in CGA-treated groups compared to the I/R group.
RT-PCR also revealed significantly lower TGF-β1 and vimentin mRNA expressions with higher SOD-1 mRNA expression.
CGA-treated groups also demonstrated a significantly lower macrophage and myofibroblast number compared to the I/R group.
These findings associated with lower mRNA expression of TLR-4, TNF-α, NF-κB, and MCP-1 as inflammatory mediators in CGA groups.
I/R + CGA3 represented the highest amelioration effect among other CGA-treated groups.
CGA treatment attenuates kidney I/R injury through reducing inflammation, decreasing myofibroblast expansion, and inducing epithelial cells proliferation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Arfian, Nur& Wahyudi, Danny A. P.& Zulfatina, Ingesti B.& Citta, Arsitya N.& Anggorowati, Nungki& Multazam, Ali…[et al.]. 2019. Chlorogenic Acid Attenuates Kidney IschemicReperfusion Injury via Reducing Inflammation, Tubular Injury, and Myofibroblast Formation. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126022
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Arfian, Nur…[et al.]. Chlorogenic Acid Attenuates Kidney IschemicReperfusion Injury via Reducing Inflammation, Tubular Injury, and Myofibroblast Formation. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1126022
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Arfian, Nur& Wahyudi, Danny A. P.& Zulfatina, Ingesti B.& Citta, Arsitya N.& Anggorowati, Nungki& Multazam, Ali…[et al.]. Chlorogenic Acid Attenuates Kidney IschemicReperfusion Injury via Reducing Inflammation, Tubular Injury, and Myofibroblast Formation. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126022
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126022
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر